Z-DNA binding protein and applications

Details Z-DNA binding protein and applications Z-DNA binding protein and applications

1995-06-20

1998-03-10

Rories, Charles C. P.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 6, 435 721, 435 691, 435 697, 435 914, 4351721, 435196, 435212, 4353201, 435375, 536 231, 536 232, 536 234, 536 235, 536 241, 514 44, C12N 1585, C12N 1563, C12N 1562, C12N 510

Patent

active

057260508

ABSTRACT:
Disclosed is the isolation of a 140,000 kDa protein which binds with high affinity to Z-DNA. Z-DNA is defined herein as a non-B-DNA conformer which is stabilized by negative supercoiling. The isolated protein also has a binding site for double-stranded RNA (dsRNA). Peptide sequences from this protein show similarity to double-stranded RNA adenosine deaminase (dsrad), an enzyme which deaminates adenosine in dsRNA to form inosine. Assays for this enzyme confirm that dsrad activity and Z-DNA binding are properties of the same molecule. The coupling of these two activities in a single molecule indicate a novel mechanism of gene regulation which is in part dependent on DNA topology.

REFERENCES:
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Kim et al., Proc. Natl. Acad. Sci. USA 91: 11457 (1994).
O'Connell et al., Molec. & Cell. Biol. 15: 1389 (1995).

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