Tissue culture of plant material enriched in secondary...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

Reexamination Certificate

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Reexamination Certificate

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10430591

ABSTRACT:
Plant material enriched in secondary metabolites is produced in tissue culture under conditions that organogenically produce a proliferation of shoots and leafy material, and harvesting the leafy material and shoots while in a green, actively-growing, non-senescent stage. Of particular interest is plant material that produces alkaloids, especially material from poppy (Papaversp.) that will yield economically attractive levels of morphinane alkaloids, particularly thebaine.

REFERENCES:
patent: 6060314 (2000-05-01), Tisserat et al.
Ilahi, I et al., Regenerating in cultures ofPapaver bracteatumas influenced by growth hormones and temperature, 1994, Plan Cell, Tissue and Organ Culture (38) p. 81-83.
Tisserat, B et al., “Influence of Ultra-high Levels of Carbon Dioxide on Secondary Metabolite Production in Vitro,” Jun. 1999, HortScience, vol. 34(3), #563, p. 543.
Hirata, K. et al., “Production of Indole Alkaloids in Multiple Shoot Culture ofCatharanthus roseus(L). G. Don.” Agric. Biol. Chem., 1987, 51 (5), p. 1311-1317.
Margaret F. Roberts, Isoquinolines (PapaverAlkaloids), Cell Culture and somatic Cell Genetics of Plants, Academic Press, Inc., vol. 5, pp. 315-334 (1998).
T. Lorenz et al., Production of Morphine Alkaloids: (S)-norlaudanosoline, a Key Intermediate, Enzyme Microb. Technol., vol. 10, pp. 219-226 (1988).
Kathryn K. Kamo et al., Morphinane Alkaloids in Cultured Tissues and Redifferentiated Organs ofPapaver Somniferum, Phytochemistry, vol. 21, No. 1, pp. 219-222 (1982).
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Abdelmajid Kassem et al., Somatic Embryogenesis, Rhizogenesis, and Morphinan Alkaloids Production in Two Species of Opium Poppy, J. Biomedicine and Biotechnology, 1:2, pp. 70-78 (2001).
K.B. Day et al., Plant Regeneration and thebaine Content of Plants Derived from Callus Culture ofPapaver bracteatum, Plant Cell Reports, 5:471-474 (1986).
E.G. Alkhimova et al., Production of medicinal Alkaloids byPapaver Bracteatum Cultured Cells, Acta Horticulturae 330, Medicinal and Aromatic Plants, pp. 287-288 (1993).
Brent Tisserat, Growth Responses and Construction Costs of Various Tissue Culture Systems, Technology & Product Reports, Hort Technology, pp. 62-68, Jan./Mar. 1996.
Samuel Galewsky, Synthesis of Morphinane Alkaloids During Opium Poppy Somatic Embryogenesis, Plant Science, 45:215-222 (1986).

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