Spectrally resolvable rhodamine dyes for nucleic acid sequence d

Details Spectrally resolvable rhodamine dyes for nucleic acid sequence d Spectrally resolvable rhodamine dyes for nucleic acid sequence d

1989-09-29

1994-11-22

Low, Christopher S. F.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 2532, C12Q 168

Patent

active

053668600

ABSTRACT:
A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucleotides used in the sequencing method by way of an alkynylamino linker. Preferably, the labeled dideoxynucleotides are incorporated into the growing DNA chains by Taq DNA polymerase.

REFERENCES:
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Chem. Abstr. vol. 108, No. 23, Sproat, 1987, Abstr. No. 199501r.
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Connell et al., Biotechniques, vol. 5, pp. 342-348 (1987).
Karger et al., Nucleic Acids Research, vol. 19, pp. 4955-4962 (1991).
Wehry, Chapters 3 and 4, in Guilbault, editor, Practical Fluorescence, 2nd Ed. (Marcel Dekker, New York, 1990).
Haugland chapter 2, in Steiner, editor, Excited States of Biopolymers (Plenum Press, New York, 1983).
Haugland, Handbook of Fluorescent Probes and Research Chemicals, (Molecular Probes, Inc., Eugene, 1989) excerpts showing emission max.
Kodak Laboratory Chemicals Catalog 1981-1982 (Eastman Kodak Co., Rochester, 1981) excerpts showing emission max of fl. dyes.

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