Recombinant sigma NS protein

Details Recombinant sigma NS protein Recombinant sigma NS protein

1985-11-08

1988-08-16

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4351723, 435948, 436501, 935 77, 935 78, C12Q 168, G01N 33566, C12N 1500

Patent

active

047644605

ABSTRACT:
This disclosure relates to the synthesis by application of recombinant DNA technology of recombinant reovirus nonstructural protein sigma NS and its use as a non-specific binding agent for single stranded (ss) RNA's. This property of sigma NS protein can be employed when it is used as a reagent in protecting unstable RNA during extraction processes and more significantly as a reagent for the concentration of (ss) RNA samples for use in hybridization probe assays.

REFERENCES:
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Encarnacion Martinez-Salas, Juan Ortin and Esteban Domingo Gene 35, 55-61, 1985, "Expression of a Functional Influenza Viral Cap-Recognizing Protein by using a Bovine Papilloma Virus Vetor".
Janet Braam-Markson, Carol Jaudon, and Robert M. Krug Proc. Natl. Acad. Sci. USA 82, 4326-4330, Jul. 1985.
Huismans, J. and Joklik, W., "Reovirus Coded Polypeptides in Infected Cells: Isolation of Two Native Monomeric Polypeptides with Affinity for Single-Stranded and Double-Stranded RNA, Respectively," Virology, 70, 411-424, 1976.

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