Protein complexes having factor VIII:C activity and production t

Details Protein complexes having factor VIII:C activity and production t Protein complexes having factor VIII:C activity and production t

1994-06-27

1998-08-04

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4352402, 4353201, 530383, 536 235, 930100, C07K 14755, C12N 510, C12N 1512, C12N 1563

Patent

active

057892032

ABSTRACT:
Recombinant protein complexes having human Factor VIII:C activity are expressed in a eukaryotic host cell by transforming the host cell with first and second expression cassettes encoding a first polypeptide substantially homologous to human Factor VIII:C A domain and a second polypeptide substantially homologous to human Factor VIII:C C domain, respectively. In the present invention, the first polypeptide may be extended having at its C-terminal a human Factor VIII:C B domain N-terminal peptide, a polypeptide spacer of 3-40 amino acids, and a human Factor VIII:C B domain C-terminal peptide. Expression of the second polypeptide is improved by employing an .alpha..sub.1 -antitrypsin signal sequence.

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