Process for measuring complement activity and reagent used there

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 23, 435 24, 435966, 435967, 436518, 436528, 436821, 436829, G01N 3353

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active

060156791

ABSTRACT:
A reagent composition comprising (a) liposomes encapsulating a marker therein, immobilizing a hapten on liposome membranes and having a size in the range of 100 to 500 nm in terms of mean particle size plus twice the standard derivation, and (b) an antibody to the hapten is effective for measuring human complement activity easily and precisely with excellent storage stability.

REFERENCES:
Kinsky et al., Complement-Dependent Damage to Liposomes Prepared from Pure and Forssman Hapten, "Biochemistry" vol. 8, No. 10, pp. 4149-4158, (1969).
Geiger et al., Immunochemical Determination of Ganglioside GM, By Inhibition of Complement-Dependent Liposome Lysis, "Journal of Immunological Methods," 17, pp. 7-19, (1977).
Bowden et al., Homogeneous, Liposome-Based Assay for Total Complement Activity in Serum, "Clinical Chemistry," vol. 32, No. 2, pp. 275-278, (1986).
Derwent Publications Ltd., GB; JP A 01 155 271--Jun. 19, 1989, Abstract.
Patent Abstracts of Japan; Tomioka Kanju et al., Jun. 7, 1994, JP A 6 160 390--Abstract.
Patent Abstracts of Japan; Kanegafuchi Chem Ind. Co. Ltd., Sep. 13, 1989, JP-A-01 229 970--Abstract.
BiochImica et Biophysica Acta; "Biomembranes," vol. 557, No. 1, Oct. 19, 1979 Amsterdam, p. 9-23.
Masaki et al., J. Imuno. Methods, 123(1), p. 19-24, Sep. 29, 1989.
Paul et al., "Fundamental Immunology," 1985 by Raven Press, NY, NY, p. 680.
Sigma Chemical Company Catalogue, 1992, pp. 282-283.
Vance et al., "The Biochemistry of Lipids and Membranes," 1985 by Benjamin/Cummings Publishing Co., Inc., California, pp. 36-39.

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