Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-11-23
1996-11-05
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 18, 435 911, 435 912, 536 2532, C07H 2104, C12P 1934, C12Q 168
Patent
active
055716733
ABSTRACT:
The present invention provides methods of detecting a change in the length of an oligonucleotide labeled with a light-emitting label by measuring the light emission in the presence of a DNA binding compound that interacts with the label to modify the light emission of the label, wherein the degree of interaction depends on the length of the oligonucleotide. The methods are applicable in general to nucleic acid sequence detection assays that utilize a reaction that results in the selective cleavage of hybridized oligonucleotide probes, and, in particular, to amplification/detection assays wherein hybridized probes are cleaved concomitant with primer extension.
REFERENCES:
patent: 5210015 (1993-05-01), Gelfand et al.
Higuchi et al., Nuc. Acids Res. 17(14):5865, 1989.
Kingsbury et al. "Rapid Detection and Identification of Infectious Agents, " pp. 245-256, 1985.
Morrison, Nonisotopic DNA Probe Techniques, Academic Press, Inc., 1992, Chapter 13, entitled "Detection of Energy Transfer and Fluorescence Quenching".
Gould George M.
Hoffmann-La Roche Inc.
Jones W. Gary
Petry Douglas A.
Tramaloni Dennis P.
LandOfFree
Methods for in-solution quenching of fluorescently labeled oligo does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for in-solution quenching of fluorescently labeled oligo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for in-solution quenching of fluorescently labeled oligo will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2014216