Method for the determination of the enzymatic activity of conver

Details Method for the determination of the enzymatic activity of conver Method for the determination of the enzymatic activity of conver

1987-10-30

1990-01-09

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 18, 435 23, 435 24, 435212, 435219, C12Q 100, C12Q 134, C12Q 136, C12Q 138

Patent

active

048928135

ABSTRACT:
The invention relates to a method for measuring the enzymatic activity of a convertase, wherein:

REFERENCES:
Clamagirand et al., Biochem. Biophys. Res. Commun (1986) 134:1190.
Cohen, Biochimie (1987) 69:87-89.
Gluschankof et al., J. Biol. Chem. (1987) 262:1-6.
Gomez et al., "The Somatostatin-28 Convertase of Rat Brain Cortex is Associated with Secretory Granule Membranes." J. Biol Chem. vol. 260, No. 19, pp. 10541-10545 (Sep. 5, 1985).
Gluschankof, et al., "The somatostatin-28 Convertase of Rat Brain Cortex Generates Both Somatostatin-14 and Somatostatin-28 (1-12)" Biochem. and Biophys. Res. Comm. vol. 128, No. 3, May 16, 1985, pp. 1051-1057.
Gluschankof et al., "Enzymes Processing somatostatin precursors: An Arg-Lys esteropeptidase . . . " Proc. Natl. Acad Sci USA, vol. 81, pp. 6662-6666 (Nov. 1984).
Ruggere et al., "Hepatic Metabolism of Somatostatin-14 and Somatostatin-28: Immunochemical Characterization of the Metabolic Fragments and Comparison of Cleavage Sites", Endocrinolgy vol. 117, No. 1, pp. 88-96.
Akopian, et al. Vopr Biokhim Mozsa, 1978, 13 pp. 189-205 (abstract only).

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