Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Reexamination Certificate
2011-06-28
2011-06-28
Saidha, Tekchand (Department: 1652)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
C435S100000, C435S194000
Reexamination Certificate
active
07968309
ABSTRACT:
A sucrose phosphorylase (SP) having improved thermostability obtained by modifying a natural SP and a method for producing the SP having improved thermostability is provided. This SP having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase, in at least one position selected from the group consisting of a position corresponding to position 14, a position corresponding to position 29 and a position corresponding to position 44 in motif sequence 1; a position corresponding to position 7, a position corresponding to position 19, a position corresponding to position 23 and a position corresponding to position 34 in motif sequence 2; and a position corresponding to position 19 in motif sequence 3, and wherein the enzyme activity of the SP having improved thermostability at 37° C., after heating the SP having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C. for 20 minutes, is 20% or more of enzyme activity of the SP having improved thermostability at 37° C. before heating.
REFERENCES:
Tettelin et al Science 293, 498-506, 2001.
International Search Report for corresponding Application No. PCT/JP2004/012533 mailed Dec. 28, 2004.
Pimental M.C.B. et al., “Screening Thermal Properties and Production in Yam Extract of Fungal Sucrose Phosphorylase”, Revista de Microbiologia, 1992, vol. 23, pp. 199 to 205.
Lehmann M. et al., “Engineering Proteins for Thermostability; the Use of Sequence Alignments Versus Rational Design and Directed Evolution”, Current opinion in Biotechnology, 2001, vol. 12, pp. 371 to 375.
Fujii Kazutoshi
Iiboshi Masae
Kuriki Takashi
Takaha Takeshi
Takata Hiroki
Ezaki Glico Co., Ltd.
Renner , Otto, Boisselle & Sklar, LLP
Saidha Tekchand
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