Isolated DNA encoding enzyme for phage resistance

Food or edible material: processes – compositions – and products – Fermentation processes – Of milk or milk product

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4351723, 4352523, A23C 913, C12N 1587

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active

059253886

ABSTRACT:
An isolated DNA of a Lactococcus lactis showing a SEQ ID NO:1 encoding a restriction and twp modification enzymes (R/M SEQ ID NO: 2, 3 and 4). The isolated DNA is used to transform sensitive dairy cultures, such as Lactococcus lactis and Streptococcus thermophilus, to provide phage resistance. Escherichia coli can be used to produce endonucleases.

REFERENCES:
Mercenier, A. et al. "Genetics of Streptoccus thermophilus: a review" Journal of Dairy Science (Dec. 1989), vol. 72, No. 12, pp. 3444-3454.
Nyengaard, N. et al. "LlaAl and LlaBl, two type-II restriction endonucleases from Lactoccus lactis subsp. cremoris W9 and W56 recognizing, respectively, 5'-/GATC-3' and 5'-C/TRY AG-3'" Gene (Dec. 22, 1993), vol. 136, No. 1-2, pp. 371-372.

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