Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1996-06-04
1998-12-01
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435195, 435196, 435 71, 435 76, 435 79, 530402, 536 232, C12N 916, C12N 1555, C12Q 144, C07K 1900
Patent
active
058436340
ABSTRACT:
This invention relates to genetically engineered enzymes, their ligand conjugates, their manufacture, and their use in qualitative or quantitative assays. A hybrid enzyme, such as an AP-epitope, has a foreign amino acid moiety (an epitope) inserted near the active site of the starting AP enzyme. The foreign amino acid moiety binds with an analyte, and, as a consequence of this binding, the enzymatic activity of the hybrid enzyme, AP-epitope, is modified. The changes in the enzymatic activity are dependent upon the presence, or the amount, of the analyte. In another embodiment, the hybrid enzyme consists of a cysteine introduced near the active site of an AP to give a hybrid enzyme. The cysteine on the hybrid enzyme serves as a point of conjugation of a ligand, such as theophylline, ferritin, thyroxine, or digoxigenin, to form the hybrid enzyme-ligand conjugate. The ligand binds with an antibody, an analyte or a binding molecule to an analyte and as a result of this binding, the enzymatic activity of the hybrid enzyme-ligand conjugate is modified or modulated.
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Brate Elaine M
Brennan Catherine A.
Bridon Dominique P.
Jaffe Keeve D.
Krafft Grant A.
Abbott Laboratories
Danckers Andreas M.
Moore William W.
Wax Robert A.
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