Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1997-11-21
2000-11-07
Nashed, Nashaat T.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435187, 435189, 435195, 435197, C12N 916, C12N 998
Patent
active
061435430
ABSTRACT:
An enzyme system is described that is useful for preparing food and feed. One enzyme of that system is obtainable from Aspergillus. That enzyme has the following characteristics: a MW of from 29 kDa to 36 kDa as measured on a SDS-Phastgel (10-15%) or about 30 kDa; a pI value of about 3-4; ferulic acid esterase activity; a pH optimum of about 5; and a temperature optimum of from about 50 to about 60.degree. C.
REFERENCES:
patent: 5707847 (1998-01-01), Christgau et al.
de Vries et al. "The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterase involved in degradation of complex cell wall polysaccharides" App. Environ. Microbiol. 63 (12), 4638-4644, Dec. 1997.
Faulds et al. "Purification and characterization of a ferulic acid esterase (FAE-III) from Aspergillus niger: specificity for the phenolic moity to microcrystalline cellulose" Microbiology 140, 799-787, 1994.
Faulds et al. "Ferulic acid esterase from Aspergillus niger: purification and partial characterization of two forms from commercial source of pectinase" Biotechnol. App. Biochem. 17, 349-359, Jun. 1993.
Graf, E. "Antoxidant potential of ferulic acid" Free Radical Biol. Med. 13, 435-448, 1992.
Ralet et al. "Degradation of feryloylated oligosaccharides from sugar-beet pulp and wheat bran by ferulic acid esterase from Aspergillus niger" Carbohyd. Res. 263, 257-269, 1994.
Faulds et al. "Release of ferulic acid from plant polysaccharides by ferulic acid esterase from Streptomyces olivochromogenes," Carbohydrate Polymers 21:153-55 (1993).
Faulds et al. "Ferulic acid release from plant polysaccharides by specific esterases," in Xylans and Xylanases (ed by J. Visser et al., Elseveier Science Publishers 1992).
De Vries Ronald Peter
Michelsen Birgit
Poulsen Charlotte Horsmans
S.o slashed.e J.o slashed.rn Borch
Visser Jacob
Danisco A/S
Kowalski Thomas J.
Nashed Nashaat T.
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