E6 associated protein and methods of use thereof

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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4352351, C07H 2104

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active

055323480

ABSTRACT:
The present invention provides compositions of isolated and purified E6 Associated Protein and fragments thereof. Also provided are nucleic acid constructs encoding E6 Associated Protein. These compositions may be employed to identify compounds which inhibit binding of high risk HPV E6 to p53. The compositions of the present invention may also be used in methods to detect the presence of high risk HPV in biological samples.

REFERENCES:
AA. Huibregtse, et al., "Cloning and Expression of the cDNA for E6-AP, a Protein That Mediates the Interaction of the Human Papillomavirus E6 Oncoprotein with p53" Mol. Cell. Biol. 13(2):775-84 (Feb. 1993).
AB. Huibregtse, et al., "Localization of the E6-AP Regions That Direct Human Papillomavirus E6 Binding, Assocation with p53, and Ubiquitination of Associated Proteins" Mol. Cell. Biol. 13(8): 4918-4927 (Aug. 1993).
Oren et al., "Post-Translational Regulation of the 54K Cellular Tumor Antigen in Normal and Transformed Cells", Mol. Cell. Biol., 1:101-110 (1981).
zur Hausen and Schneider, "The Role of Papillomaviruses in Human Anogential Cancer", in The Papovavirides, vol. 2, Plenum, New York, 1987.
Schlegel et al., "Quantitative keratinocyte assay detects two biological activities of human papillomavirus DNA and identifies viral types associated with cervical carcinoma", EMBO J., 7:3181-3187 (1988).
Watanabe et al., "Human Papillomavirus Type 16 Transformation of Primary Human Embryonic Fibroblasts Requires Expression of Open Reading Frames E6 and E7", J. Virol., 63:965-969 (1989).
Munger et al., "Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product", EMBO J., 8:4099-4105 (1989).
Finlay et al., "The p53 proto-Oncogene Can Act as a Suppressor of Transformation", Cell, 57:1083-1093 (1989).
Eliyahu et al., "Wild-type p53 can inhibit oncogene-mediated focus formation", Proc. Natl. Acad. Sci. USA, 86:8763-8767 (1989).
Lane and Benchimol, "p53: Oncogene or Anti-oncogene?" Genes and Devel., 4:1-8 (1990).
Werness et al., "Association of Human Papillomarvirus Types 16 and 18 E6 Proteins with p53," Science, 248:76-79 (Apr. 6, 1990).
Vogelstein, "A Deadly Inheeritance," Nature, 348:681-682 (Dec., 1990).
Malkin et al., "Germ Line p53 Mutations in a Familial Syndrome of Breast Cancer, Sarcomas, and Other Neoplasms," Science, 250:1233-1238 (Nov. 30, 1990).
Srivastava et al., "Germ-line transmission of a mutated p53 gene in a cancer-prone family with Li-Fraumeni syndrome," Nature, 348:747-749 (Dec., 1990).
Diller et al., "p53 Functions as a Cell Cycle Control Protein in Osteosarcomas", Mol. Cell. Biol., 10:5772-5781 (1990).
Werness et al., "Association of Human Papillomavirus Types 16 and 18 E6 Proteins with p53", Science, 248:76-79 (Apr. 6, 1990).
Scheffner et al., "The E6 Oncoprotein Encoded by Human Papillomavirus Types 16 and 18 promotes the Degradation of p53", Cell, 63:1129-1136 (1990).
Baker et al., "Suppression of Human Colorectal Carcinoma Cell Growth by Wild-Type p53," Science, 249:912-915 (Aug. 24, 1990).
Huibregtse et al., "A Cellular Protein Mediates Association of p53 with the E6 Oncoprotein of Human Papillomavirus Types 16 or 18", EMBO J., 10:4129-4135 (1991).
Wrede et al., "Expression of RB and p53 Proteins in HPV-Positive and HPV-Negative Cervical Carcinoma Cell Lines", Mol. Carcin., 4:171-175 (1991).
Scheffner et al., "The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines", Proc. Natl. Acad. Sci. USA, 88:5523-5527 (1991).
Martinez et al., "Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein", Genes and Devel., 5:151-159 (1991).
Levine et al., "The p53 tumour suppressor gene," Nature, 351:453-456 (Jun. 6, 1991).
Ginsberg et al., "Wild-type p53 can down-modulate the activity of various promoters", Proc. Natl. Acad. Sci. USA, 88:9979-9983 (1991).
Fakharzadeh et al., "Tumorigenic potential associated with enhanced expression of a gene that is amplified in a mouse tumor cell line", EMBO J., 10:1565-1569 (1991).
Oliner et al., "Amplification of a Gene Encoding a p53-associated Protein in Human Sarcomas", Nature, 358:80-83 (Jul. 2, 1992).
Momand et al., "The mdm-2 Oncogene Product Forms a Complex with the p53 Protein and Inhibits p53-Mediated Transactivation", Cell, 69:1237-1245 (1992).
Kern et al., "Oncogenic Forms of p53 Inhibit p53-Regulated Gene Expression," Science, 256:827-830 (May 8, 1992).
Farmer et al., "Wild-type p53 activates transcription in vitro," Nature, 358:83-86 (Jul. 2, 1992).
Huibregtse et al., Mol. Cell. Biol. 13(2):775-784, Feb. 1983.
Prives et al., Genes Dev., 7(4):529-534, Apr. 1993.

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