Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1996-09-23
1998-09-15
Hutzell, Paula K.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530412, 5303871, 5303881, 5303882, 5303886, A23J 100, C07K 1600
Patent
active
058080090
ABSTRACT:
The present invention relates to a method of affinity purifying antibodies consisting of the following steps: A) homogenizing Helminth parasite worms forming a mixture; B) incubating the mixture of adult Helminth parasite worm homogenate with pre-swelled CNBr-activated Sepharose 4B in coupling buffer; C) first washing the mixture with blocking buffer; D) second washing the mixture with acetate buffer and coupling buffer; E) third washing the mixture with Phosphate Buffered Solution; F) pouring the mixture into a chromatography column; G) allowing the mixture to settle; H) first passing, by gravity, a solution of saturated ammonium sulfate precipitated antibodies in loading buffer through the chromatography column; I) second passing, by gravity, loading buffer through the chromatography column until thoroughly washed; and J) second passing, by gravity, chromatography column elution buffer through the chromatography column which functions to remove and the purified antibodies.
REFERENCES:
Robinson et al. (1994) Cellular Immunology vol. 158, 157-166.
England et al. (1990) Methods in Enzymology vol. 182, 285-300.
Cuatrecasas et al (1990) Methods in Enzymology vol. 22, 345-385.
Gustad Thomas Russell
Meinhardt Steven Wayne
Robinson Michael
Hutzell Paula K.
Lingbeck David A.
Masood Khalid
North Dakota State University Research Foundation
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