Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Reexamination Certificate
2004-03-06
2010-12-28
Ulm, John D (Department: 1649)
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
C435S007100, C435S007200, C435S007210, C435S008000, C435S363000
Reexamination Certificate
active
07858394
ABSTRACT:
The invention relates to methods for the quantitative optical analysis of the intracellular concentration of the cyclic nucleotides cGMP and cAMP, said methods using cell lines which express a combination of certain CNG channels, a calcium-sensitive photoprotein, and different target proteins for which modulators are to be found, in a recombinant manner. The cell lines modified in this way are suitable for high-throughput screening (HTS and uHTS) and can be used to identify medicaments which influence the activity of receptors or enzymes participating in the composition or decomposition of the cyclic nucleotides cGMP and cAMP.
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Sautter, A., et al., “An isoform of the rod photoreceptor cyclic nucleotide-gated channel beta subunit expressed in olfactory neurons”, Proc. Natl. Acad. Sci. USA, 95: 4696-4701 (Apr. 1998).
Axxam SpA
DeCloux Amy
Edwards Angell Palmer & & Dodge LLP
Loren Ralph A.
Ulm John D
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