Process for producing kiss-1 peptide

Details Process for producing kiss-1 peptide Process for producing kiss-1 peptide

2005-01-04

2005-01-04

McKelvey, Terry (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C530S300000, C530S350000, C530S407000

Reexamination Certificate

active

06838259

ABSTRACT:
A KiSS-1 peptide or a salt thereof can be produced in a large scale by subjecting a fusion protein or peptide in which a KiSS-1 peptide is ligated to the N-terminal of a protein or peptide having cysteine at the N-terminal, to the reaction of cleaving the peptide bond on the amino acid side of the cysteine residue.

REFERENCES:
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West, et al., “Chromosome Localization and Genomic Structure of the KiSS-1 Metastasis Suppressor Gene (Kiss1)”, GENOMICS 54, 145-148 (1998), Article No. GE985566.
Lee, et al., “KiSS-1, a Novel Human Malignant Melanoma Metastasis-Suppressor Gene”, Journal of the National Cancer Institute, vol. 88, No. 23, Dec. 4, 1996.
Hori, et al., “Metastin Suppresses the Motility and Growth of CHO Cells Transfected with Its Receptor”; Biochemical and Biophysical Research Communications, vol. 286, 958-963, 2001.
A Novel Procedure for the preparation of biologically active recombinant peptides using a cyanylation reaction, Koyama, N. et al, Journal of Biotechnology, vol. 32, No. 2, (1994), pp. 273-281.

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