Plant transformation and selection

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S284000, C800S294000, C800S298000

Reexamination Certificate

active

07960614

ABSTRACT:
The present invention relates to a genotype-independent method for transforming and selecting plant explants. The transformation method includes pre-culturing the explants in the presence of anAgrobacteriuminducer and exposing the transformed explants to a shoot regeneration media that accelerates shoot development. Plants generated from this transformation method are provided. In particular, methods for obtaining transgenicEucalyptusand pine cells and regenerating stably transformedEucalyptusand pine trees are provided. The invention also provides media, methods, and plasmids for selecting and regenerating plants, particularly forest trees.

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