Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1996-07-30
2000-01-25
Crouch, Deborah
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 231, 4353201, C07H 2104, C07H 2102, C12N 1500
Patent
active
060180390
ABSTRACT:
This invention relates to a process for producing a transgenic animal wherein gene expression regulatory region for a gene from chromosomal DNA is introduced in the animal to enable the expression of the desired exogenous gene in the animal. More specifically, this invention relates to a process in the field of transgenic animal technology wherein expression of a desired substance in a mammal is enabled by producing a recombinant expression vector capable of amplifying in a host cell such as E. coli by using a DNA fragment including a part of the structural gene coding for mC26 protein and sequences in the upstream and downstream of the mC26 gene that regulate the expression of the mC26 gene; and introducing the gene expression regulatory region for the mC26 gene in the animal by using the thus produced expression vector to produce a model animal for the gene expression regulatory experiment, and to thereby enable the expression of the desired substance in the mammary gland or other tissue of the animal.
REFERENCES:
Nishimura, T. Direct submission to DDBJ. MPSRCH, pp. 1-4, Apr. 20, 1993.
Krimpenfort, et al. Generation of transgenic dairy cattle using `in vitro` embryo production. Biotechnology, vol. 9, pp. 844-847, Sep. 1991.
Houdebine, L. M. Production of pharmaceutical proteins from transgenic animals. J. of Biotechnology, vol. 34, pp. 269-287, 1994.
Pursel et al. Genetic enginerring of Livestock. Science, vol. 244, pp. 1281-1288, Jun. 16, 1989.
Satow et al., "Post-Transcriptional Control of 26 K Casein Genes during Lactogenesis in Mouse Mammary Glands", J. Biochem. 99, vol. 99, No. 6, pp. 1639-1643 (1986).
Kawamura et al., "Modulation of the Transferred Mouse 26K Casein Gene in Mouse L Cells by Glucocorticoid Hormone", J. Biochem. 101, No. 1, pp. 103-110 (1987).
Nishimura et al., "Expression of the MC26 Gene Encoding GlyCAM-1 in the Lactating Mouse Mammary Gland", J. Biochem. vol. 114, No. 4, pp. 567-569 (1993).
Dowbenko et al., "Structure and Chromosonal Localization of the Murine Gene Encoding GLYCAM-1", The Journal of Biological Chemistry vol. 268, No. 6, pp. 4525-4529 (1993).
Dowbenko et al., "Glycosylation-dependent Cell Adhesion Molecular 1 (GlyCAM 1) Mucin Is Expressed by Lactating Mammary Gland Epithelial Cells and is Present in Milk", J. Clin. Invest. vol. 92, Aug. 1993, pp. 952-960.
Lasky et al., "An Endothelial Ligand for L-Selectin Is a Novel Mucin-like Molecule", Cell. vol. 69, Jun. 12, 1992, pp. 927-938.
Imai et al., "Sulphation requirement for GlyCAM-1, an endothelial ligand for L-selectin", Nature vol. 361, Feb. 11, 1993, pp. 555-557.
Grusby et al., "Casein expression in cytotoxic T lymphocytes", Proc. Natl. Acad. USA vol. 87, Sep. 1990, pp. 6897-6901.
Ebert et al., "Transgenic Production of a Variant of Human Tissue-Type Plasminogen Activator in Goat Milk: Generation of Transgenic Goats and Analysis of Expression", Bio/Technology vol. 9, No. 9, Sep. 1991, pp. 835-838.
Denman et al., "Transgenic Expression of a Variant of Human Tissue-Type Plasminogen Activator in Goat Milk: Purification and Characterization of the Recombinant Enzyme", Bio/Technology vol. 9, No. 9, Sep. 1991, pp. 839-843.
Clark Deborah J. R.
Crouch Deborah
Pepper Frederick W.
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