Convergent synthesis of branched and multiply connected macromom

Details Convergent synthesis of branched and multiply connected macromom Convergent synthesis of branched and multiply connected macromom

1995-05-31

1996-11-05

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 911, 435 912, 435 71, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168, C12Q 170, C12P 1934, C07H 2104

Patent

active

055716776

ABSTRACT:
The invention provides methods and compositons for convergent synthesis of branched polymers useful as molecular probes. The invention also includes several novel branched polymeric structures particularly useful for detecting target polynucleotides. Branched polymers of the invention comprise at least two branches: at least one branch is a target binding moiety capable of specifically binding to a target molecule of interest and one or more branches are signal generation moities capable of directly or indirectly generating a detectable signal. In accordance with the method of the invention branched polymers are assembled from components having phosphorothioate groups and/or haloacyl- or haloalkylamino groups. The phosphorothioate and haloacyl- or haloalkylamino groups react rapidly and efficiently when brought into contact to form thiophosphorylacyl- or thiophosphorylalkylamino bridges which.complete the assembly of a branched polymer. The method of the invention permits thorough purification and isolation of the components prior to final assembly. Incomplete branched polymers are readily separated from the desired product by standard means. The method of the invention permits the synthesis of several novel branched polymer configurations.

REFERENCES:
patent: 4775619 (1988-10-01), Urdea
patent: 5245022 (1993-09-01), Weis et al.
patent: 5256775 (1993-10-01), Froehler
patent: 5473060 (1995-12-01), Gryaznov et al.
patent: 5476930 (1995-12-01), Letsinger
S. Agrawal et al., "Oligodeoxynucleoside phosphoramidates and phosphorothioates as inhibitors of human immunodeficiency virus", Proc. Natl. Acad. Sci. USA, vol. 85, pp. 7079-7083, 1988.
C. Chang et al., "Improved methods for the synthesis of branched DNA (bDNA) for use as amplification multimers in bioassays", Nucleosides & Nucleotides, vol. 10(1-3), pp. 389-392, 1991.
J. M. Dagle et al., "Physical properties of oligonucleotides containing phosphoramidate-modified internucleoside linkages", Nucleic Acids Research, vol. 19 (8), pp. 1805-1810, 1991.
N. Dolinnaya et al., "Construction of branched DNA (bDNA) molecules by chemical ligation", Bioorganic & Medicinal Chemistry Letters, vol. 4 (8), pp. 1011-1018, 1994.
J. C. Francois et al., "Periodic cleavage of poly(dA) by oligothymidylates covalently linked to the 1,10-phenanthroline-copper-complex", Biochemistry, vol. 27, pp. 2272-2276, 1988.
M. A. Grachev et al., "A route to RNA with an alkylating group at the 5'-triphosphate residue", Nucleic Acids Research, vol. 8(15), pp. 3413-3426, 1980.
R. R. Hodges et al., "`Post-assay` covalent labeling of phosphorothioate-containing nucleic acids with multiple flourescent markers", Biochemistry, vol. 28, pp. 261-267, 1989.
T. Horn et al., "Solid supported chemical 5'-phosphorylation of oligodeoxy-ribonucleotides that can be monitored by trityl cation release: application to gene synthesis", Nucleosides & Nucleotides, vol. 6 (1-2), pp. 335-340, 1987.
T. Horn et al., "The synthesis of branched oligonucleotides as signal amplification multimers for use in nucleic acid assay", Nucleosides & Nucleotides, vol. 8 (5-6), pp. 875-877, 1989.
T. Le Doan et al., "Sequence-specific recognition, photocrosslinking and cleavage of the DNA double helix by an oligo-[.alpha.]-thymidylate covalently linked to an azidoproflavine derivative", Nucleic Acids Research, vol. 15(19), pp. 7749-7760, 1987.
J. F. Milligan et al., "Current concepts in antisense drug design", Journal of Medicinal Chemistry, vol. 36(14), pp. 1923-1937, 1993.
S. I. Oshavski, "Enzymatic transfer of ATP.gamma.S thiophosphate onto the 5'hydroxyl of an oligonucleotice as a route to reactive oligonucleotide derivates", FEBS Letters, vol. 143(1), pp. 119-123, 1982.
D. Praseuth et al., "Sequence-specific binding and photocrosslinking of .alpha. and .beta. oligodeoxynucleotides to the major grove of DNA via triple-helix formation", Proc. Natl. Acad. Sci. USA, vol. 85, pp. 1349-1353, 1988.
Horn et al, Nucleic Acids Research 17: 6959-6967.
Goodchild et al., Bioconj. Chem. 1: 165-187.
Fidanza et al. J. Am Chem Society 111: 9117-9119.

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