Production of human somatomedin C

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 691, 435 694, 4351723, 4352523, 4352542, 4353201, 536 231, 536 234, 536 2351, C12N 1562, C12N 1516, C12N 1574, C12N 1581

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054707218

ABSTRACT:
A process for selecting DNA sequences that are optimal for the production of polypeptides in hosts transformed with those DNA sequences. These DNA sequences, which code for a variety of human and animal proteins, permit the high level expression of those products in host cells. In the preferred embodiment of this invention, DNA sequences optimal for the production of human somatomedin C are selected and employed to express that growth enhancing factor.

REFERENCES:
patent: 4738921 (1988-04-01), Belagaje et al.
patent: 5019500 (1991-05-01), Ueda et al.
patent: 5028531 (1991-07-01), Ueda et al.
D. Gheysen et al., "Systematic Alteration of the Nucleotide Sequence Preceding the Translation Initiation Codon and the Effects on Bacterial Expression of the Cloned SV40 Small-t Antigen Gene", Gene, 17, pp. 55-63 (1982).
R. Grantham et al., "Codon Frequencies In 119 Individual Genes Confirm Consistent Choices of Degenerate Bases According to Genome Type", Nucleic Acids Res., 8, pp. 1893-1912 (1980).
K. Itakura et al., "Chemical DNA Synthesis and Recombinant DNA Studies", Science, 209, pp. 1401-1405 (1980).
G. P. Larson et al., "Saccharomyces cerevisiae Actin-Escherichia coli lacZ Gene Fusions", Gene, 22, pp. 31-39 (1983).
Maniatis et al., "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory, p. 426 (1982).
E. Rinderknecht et al., "The Amino Acid Sequence of Human Insulin-like Growth Factor I and Its Structural Homology with Proinsulin", J. Biol. Chem. 253, pp. 2769-2776 (1978).
Takebe and Kaziro, "In Vitro Construction of the tufB-lacZ Fusion: Analysis of the Regulatory Mechanism of tufB Promoter", Mol Gen Genet, 187, pp. 355-363 (1982).
R. A. Jefferson, "-Glucuronidase From Escherichia coli As A Gene-Fusion Marker", Proc. Natl. Acad. Sci. USA, 83, pp. 8447-8451 (1986).
R. J. Kaufman and P. A. Sharp, "Consruction of A Modular Dihydrofolate Reductase cDNA Gene: Analysis of Signals Utilized for Efficient Expression", Mol. Cell Biol., 2 (11), pp. 1304-1319 (1982).
M. A. Peters et al., "Expression of a Biologically Active Analogue of Somatomedin-C/Insulin-Like Growth Factor I", Gene, 35, pp. 83-89 (1985).
E. Rinderknecht and R. E. Humbel, "Polypeptides with Nonsuppressible Insulin-Like and Cell-Growth Promoting Activities in Human Serum: Isolation, Chemical Characterization, and Some Biological Properties of Forms I and II", Proc. Natl. Acad. Sci. USA, 73, pp. 2365-2369 (1976).
L. Tessier et al., "The influence of mRNA primary and secondary structure on human IFN.sub.c gene expression in E. coli", Nucleic Acids Res., 12, pp. 7663-7675 (1984).
J. D. Watson et al., Recombinant DNA: A Short Course, pp. 176-178 (1983).
C. R. Wood, "The influence of messenger RNA secondary structure on expression of an immunoglobulin heavy chain in Escherichia coli", Nucleic Acids Res., 12, pp. 3937-3950 (1984).
T. I. Zaghloul et al., "Translational Coupling in Bacillus subtilis of a Heterologous Bacillus subtilis-Escherichia coli Gene Fusion", J. Bacteriol., 164(2), pp. 550-555 (1985).
J. L. Bennetzen and B. D. Hall, "Codon Selection in Yeast", J. Biol. Chem., 257, pp. 3026-3031 (1981).
G. Buell et al., "Optimizing the Expression in E. coli of a Synthetic Gene Encoding Somatomedin-C (IGF-1)", Nucleic Acids Res., 13, pp. 1923-1938 (1985).
L. Bulow, "Characterization Of An Artificial Bifunctional Enzyme, -Galactosidase/galactokinase, Prepared By Gene Fusion", Eur. J. Biochem., 163, pp. 443-448 (1987).
A. Carlioz et al., "Isolation of Superoxide Dismutase Mutants in Escherichia coli: Is Superoxide Dismutase Necessary for Aerobic Life?", EMBO, 5(3), pp. 632-630 (1986).
B. A. Castilho et al., "Plasmid Insertion Mutagenesis and lac Gene fusion with Mini-Mu Bacteriophage Transposons", J. Bacteriol., 158, pp. 488-495 (1984).
J. F. Delamarter et al., "Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum", EMBO Journal, 4, pp. 2575-2581 (1985).
E. J. Gren, "Recognition of messenger RNA during translational initiation in Escherichia coli", Biochimie, 66, pp. 1-29.
L. Guarente et al., "Improved Methods for Maximizing Expression of a Cloned Gene: a Bacterium That Synthesizes Rabbit Beta-Globin", Cell, 20, pp. 543-553 (1980).
K. Hall & V. Sara, "The Somatomedin Family of Polypeptide Hormones", Periodicum Biologorum, 85, Supp. 1, pp. 107-114 (1983).
D. Iserentant & W. Fiers, "Secondary Structure of mRNA And Efficiency Of Translation Initiation", Gene, 9, pp. 1-12 (1980).
Smith et al. NAR 10:4467 (1982).
Urdea et al. PNAS 80:7461 (1983).
Grosjean et al. Gene 18:199 (1982).
Carrier et al., Trends Biotechnol. 1(4):109 (1983).
Rinderknecht et al. Endocrinol. 112:2215 (1983).
Sproat et al. NAR 13:2959 (1985).

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