Protease-treated and purified cellulase compositions and methods

Details Protease-treated and purified cellulase compositions and methods Protease-treated and purified cellulase compositions and methods

1995-06-06

1997-12-23

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Textile treating

435209, 4352546, 435945, D06M 1600, C12N 942, C12N 114, C12N 100

Patent

active

057006865

ABSTRACT:
During the enzymatic "stone washing" of a denim fabric and/or garments, an undesirable redeposition of blue dye often occurs on the surfaces of the denim. The invention relates to a means of overcoming this problem using an enzyme composition comprised of Trichoderma endoglucanase and Trichoderma cellobiohydrolase that has been partially digested by a protease enzyme to separate its core and binding domains. The use of this composition reduces the redeposition of the blue dye and hence improves the stone washing process relative to using a redepositing or backstaining cellulase.

REFERENCES:
patent: 4832864 (1989-05-01), Olson
patent: 4912056 (1990-03-01), Olson
patent: 5246853 (1993-09-01), Clarkson et al.
Vesa V. Joutsjoki, "Construction by one-step gene replacement of Trichoderma reesei strains that produce the glucoamylase P of Hormoconis reesei", Current Genetics, pp. 422-429 (1994).
Ritva Saarelainen, et al., "Cloning, sequencing and enhanced expression of the Trichoderma reesei endoxylanase II (pI 9) gene x1n2", Mol Gen Genet, vol. 241, pp. 497-503 (1993).
Taina Karhunen, et al., "High frequency one-step gene replacement in Trichoderma reesei. I. Endoglucanase I overproduction", Mol Gen Genet, Vo. 241, pp. 515-522 (1993).
P.H. Nielsen, et al, "Enzyme Applications (Industrial and Therapeutic)", Encyclopedia of Chemical Technology, vol. 9, pp. 567-620 (1993).
"Ion Exchange Chromatography: Principles and Methods," Pharmacia, Laboratory Separation Division, pp. 1-71 (1988).
T.K. Ghose, "Measurement of Cellulase Activities", International Union of Pure and Applied Chemistry, vol. 59, No. 2, pp. 257-268 (1987).
D. Kochavi, et al., "Optimizing Processing Conditions in Enzymatic Stonewashing", American Dystuff Reporter, pp. 24-28 (Sep. 1990).
H. Chen, et al., "Three Forms of Cellobiohydrolase I From Trichoderma Reesei" Biochemistry and Molecular Biology International, vol. 30, No. 5, pp. 901-910 (Aug. 1993).
J. Woodward, et al., "Papain Digestion of Crude Trichoderma Reesei Cellulase: Purification and Properties of Cellobiohydrolase I and II Core Proteins", Biotechnol. Appl. Biochem., vol. 19, pp. 141-153 (1994).
H.V. Tilbeurgh, et al., "Limited Proteolysis of the Cellobiohydrolase I from Trichoderma Reesei", Separation of Functional Domains, Federation of European Biochem. Societies, vol. 204, No. 2, pp. 223-227 (Aug. 1986).
P. Tomme, et al., "Studies of the Cellulolytic System of Trichoderma Reesei QM 9414", Analysis of Domain Function in Two Cellobiohydrolases by Limited Proteolysis, Eur. J. Biochem. vol. 170, pp. 575-581 (1988).
D.A. Offord, et al., "Preparative Purification of Trichoderma Reesei Native and Core Cellobiohydrolase I by Electrophoresis and Chromatofocusing", Scientific Note, Applied Biochemistry and Biotechnology, vol. 28, No. 29, pp. 377-386 (1991).
H.C. Chen, et al., "Effect of Cellulase Size Reduction on Activity and Accessibility", Biotechnology Letters, vol. 10, No. 12, pp. 913-918 (1988).
J. Mohammad, et al., "Real-Time Kinetic Analysis of Limited Proteolysis By Ion-Exchange Chromatography Using Compressed, Non-Porous Agarose Beads", Journal of Biochemical and Biophysical Methods, pp. 41-49 (1993).
J. Woodward, et al., "Does Cellobiohydrolase II Core Protein From Trichoderma Reesei Disperse Cellulose Microfibrils?", Enzyme Microb. Technol., vol. 14, pp. 625-630 (Aug. 1992).
IndiAge Euro-L Denim-Washing Enzyme, Delivering Innovation Through Biotechnology, Genencor International (1993).
D.E. McEwan, et al., "Euro Technology", Genencor International, Inc., Proceedings of the AATCC Garment Wet Processing Symposium, pp. 1-8 (1995).
C.M.G.A. Fontes, et al, "The Resistance of Cellulase and Xylanases to Proteolytic Inactivation", Appl. Microbiol. Biotechnol., vol. 43, pp. 52-57 (1995).
S. Akiba, et al., "Effects of Size of Carbohydrate Chain on Protease Digestion of Aspergillus Niger Endo-.beta.-1,4-Glucanase", Biosci. Biotech. Biochem., vol. 59, No. 6, pp. 1048-1051 (1995).
N. Din, et al., "C.sub.1 -C.sub.x Revisited: Intramolecular Synergism in a Cellulase", Proc. Natl. Acad. Sci, USA, vol. 91, pp. 11383-11387 (Nov. 1994).
J.B. Coutinho, et al., "The Nature of the Cellulose-Binding Domain Affects the Activities of a Bacterial Endoglucanase on Different Forms of Cellulose", FEMS Microbiology Letters, vol. 113, pp. 211-218 (1993).
N. Din, et al., "Non-Hydrolytic Disruption of Cellulose Fibres by the Binding Domain of a Bacterial Cellulase", Bio/Technology, vol. 9, pp. 1096-1099 (Nov. 1991).
N.R. Gilkes, et al., "Structural and Functional Analysis of a Bacterial Cellulase by Proteolysis", The Journal of Biological Chemistry, vol. 264, No. 30, pp. 17802-17808 (Oct. 25, 1989).
N.R. Gilkes, et al., "Precise Excision of the Cellulose Binding Domains from Two Cellulomonas fimi Cellulases by a Homologous Protease and the Effect on Catalysis", The Journal of Biological Chemistry, vol. 263, No. 21, pp. 10401-10407 (Jul. 25, 1988).
M.L. Langsford, et al., "Glycosylation of Bacterial Cellulases Prevents Proteolytic Cleavage Between Functional Domains", FEBS, vol. 225, No. 1, 2, pp. 163-167 (Dec. 1987).
IndiAge Euro-L Denim-Washing Enzyme, Delivering Innovation Through Biotechnology, Genencor International (1995).

Affiliated with

Also associated with

Rating

Protease-treated and purified cellulase compositions and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Protease-treated and purified cellulase compositions and methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Protease-treated and purified cellulase compositions and methods will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1802033