Analytical element and method for theophylline determination hav

Details Analytical element and method for theophylline determination hav Analytical element and method for theophylline determination hav

1986-08-25

1989-02-21

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435184, 435805, 422 56, C12Q 142, C12N 999

Patent

active

048064700

ABSTRACT:
Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is carried out at a pH of 9 or less. The element comprises an absorbent carrier material, a suitable buffer and first and second zones in fluid contact. The isoenzyme of alkaline phosphatase and a suitable substrate for the isoenzyme are located in different zones of the element. The isoenzyme is present in one of the zones in an amount of at least about 100 I.U./m.sup.2. This level of isoenzyme reduces or eliminates bias caused by endogenous alkaline phosphatase and hemoglobin during an assay.

REFERENCES:
patent: 4264727 (1981-04-01), Kolehmainen et al.
Vinet et al., Clin. Chem., 25:8, pp. 1370-1372 (1979).
Vinet et al., Clin. Biochem., 11:2, pp. 57-61 (1978).
Fawaz et al., Z. Physiol. Chem., 353, pp. 1779-1783 (1972).
Ansari et al., Clinica Chimica Acta, 118, pp. 135-139 (1982).

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