Method for immobilizing dissolved proteins

Details Method for immobilizing dissolved proteins Method for immobilizing dissolved proteins

1988-02-16

1989-06-13

Kight, John

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

530412, 530413, 210656, C07K 1700, C12N 984, C12N 1100, C12Q 100

Patent

active

048394190

ABSTRACT:
Methods for adsorbing a protein, for example an enzyme, onto an insoluble, solid, macroporous, small-particle support by washing said support with an aqueous solution of the protein containing an electrolyte in an ionic strength of at least 0.15 mole/liter and crosslinking said protein, before, during, or after such adsorption, with a coupling component present in aqueous electrolyte-containing solution.

REFERENCES:
patent: 3767531 (1973-10-01), Olsen et al.
patent: 3983000 (1976-09-01), Messing et al.
patent: 4323650 (1982-04-01), Rosevear
patent: 4371612 (1983-02-01), Matsumoto et al.
patent: 4390626 (1983-06-01), Chibata et al.
patent: 4425434 (1984-01-01), Rosevear
patent: 4440903 (1984-03-01), Goldstein et al.
patent: 4486549 (1984-12-01), Matsomoto et al.

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