Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-08-19
1999-11-23
Kemmerer, Elizabeth
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
424 854, 424 857, 4241541, 930142, 4353723, G01N 3353, C12N 522, A61K 3821
Patent
active
059898399
ABSTRACT:
Disclosed is a method of determining the presence and activity of interferon inhibitors in a test sample, preferably human blood serum. The method includes the steps of culturing cells which express an interferon-inducible antigen in the presence of an interferon and then adding an aliquot of the cultured cells to a test sample and to a control sample. The expression of the interferon-inducible antigen is then measured in both the test sample and in the control sample and these two values compared to determine the presence and activity of interferon inhibitors in the test sample.
REFERENCES:
patent: 5215741 (1993-06-01), Young et al.
patent: 5270037 (1993-12-01), Bienzle
patent: 5474771 (1995-12-01), Lederman et al.
patent: 5503987 (1996-04-01), Wagner et al.
Gerosa et al. Different sensitivity to interleukin 4 of interleukin 2-and interferon alpha-induced CD69 antigen expression in human resting NK cells and CD3.sup.+, CD4.sup.-, CD8.sup.- lymphocytes. Cellular Immunology, (May 1992) 141 (2) 342-51.
Chorvath et al. Interferon alpha-induced modulation of leukocyte cell surface antigens: immunocytofluorometric study with human leukaemia/lymphoma cell lines. Acta Virologica, (Jan. 1991) 35 (1) 7-18.
Medenica et al. Journal of Clinical Apheresis, 9:216-221 (1994).
Chadha et al. Journal of Biological Regulators and Homeostatic Agents, 5:1-9 (Jan.-Mar. 1991).
Aszalos et al. Biochemical Medicine and Metabolic Biology, 46:267-270 (Oct. 1991).
Havredaki, M. and Barona, F. (1985) "Variations of interferon inactivators and/or inhibitors in human serum and their relationship to interferon therapy," Japan J. Med. Sci. Biol. 38: 107-111.
Kawade, Y and Watanabe, Y. (1984) "Neutralization of interferon by antibody: Appraisals of methods of determining and expressing neutralization titer," J. of Interferon Res. 4: 571-584.
Rhodes et al., (1984) "Human tumor-induced inhibition of interferon action in vitro: Reversal of inhibition by .beta.-carotene (pro-vitamin A)," Cancer Immunol. Immunother. 16: 189-192.
Bjorndahl, J.M., et al. (1988), "The 28 kDa/32 kDa activation antigen EA1; Further characterization and signal requirements for its expression," J. Immunol. 141: 4094.
J. Natl. Cancer Inst. (Bethesda) (1972), 49:891-895.
Maino, VC, Suni MA, Ruitenberg, JJ, Rapid Flow cytometric method for measuring lymphocyte subset activation, Cytometry (1995), 20(2): 127-133.
Cebrian, M. Yague, E., Rincon, M., Lopez-Botet, M., De Landazuri, M.O., Sanchez-Madrid, E., Triggering of cell proliferation through AIM, an acitvation inducer molecule expressed on activated human lymphocytes, J. Exp. Med. (1988), 168:1621-1637.
Lanier, L.L., Buck, D.W., Rhodes, L., Ding, A., Evans, E., Barney, C., Phillips, J.H., Interleukin 2 activation of natural killer cells rapidly induces the expression and phosphorylation of the Leu-23 antigen, J. Exp. Med. (1988) 1572-1585.
Testi, R., Phillips, J.H., Lanier, L.L., Leu 23 induction as an early marker of functional CD3/T cell antigen receptor triggering: requirement for receptor cross-linking, prolonged elevation of intracellular [Ca++] and stimulation of protein kinase, J. Immunol. (1989) 142:1854-1860.
Nakamura, S., Sung, S.S.J., Bjorndahl, J.M., Fu, S.M., Human T cell activation IV. T cell activation and proliferation via the early activation antigen EA-1, J. Exp. Med. (1989) 169:677-689.
Santis, A.G., Campanero, M.R., Alonso, J.L., Tugroros, A., Alonso, M.A., Yague, E., Pivel, J.P., Sanchez-Madrid, E., TNF--a production induced in T lymphocytes through the AIM/CD69 activation pathway, Eur. J. Immunol. (1992), 22:1253-1259.
Lopez-Cabrera, M., Santis, A.G., Fernandez-Ruiz, E., Blacher, R., Esch, F., Sanchez-Mateos, P., Sanchez-Madrid, E., Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CS69, a new member of the C-type animal lectin superfamily of signal transmitting receptors, J. Exp. Med. (1993), 178:537-547.
Full text of the ATCC catalog entry for MOLT 4 cell line.
Medenica Rajko D.
Powell David K.
Kemmerer Elizabeth
Romeo David S.
Shaker Ghassan I.
LandOfFree
Use of MOLT4 CD69 expression to determine the presence and activ does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Use of MOLT4 CD69 expression to determine the presence and activ, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of MOLT4 CD69 expression to determine the presence and activ will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1220313