Yeast-origin promoter and vector and expression system using...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0


C435S006120, C435S254110, C435S320100

Reexamination Certificate



The present invention provides a DNA fragment having a cold-inducible promoter function of yeast, which has high activity in a low temperature range, by identifying a DNA fragment, which exists in a non-translation region located upstream of the 5′-terminal side of a gene selected from the group consisting of cold-inducible genes ofSaccharomyces cerevisiae, and has a cold-inducible promoter function.

Nakagawa et al. Mga2p is a Putative Sensor for Low Temperature and Oxygen to Induce OLE1 Transcription inSaccharomyces cerevisiae. Biochem. and Biophys. Res. Comm. 291: 707-713. 2002.
Goldenberg et al. Role ofEscherichia colicspA promoter sequences and adaptation of translational apparatus in the cold shock response. Mol. Gen. Genet. 256: 282-290. 1997.
Lim et al. Low Temperature Reulated DEAD-box RNA Helicase from the Antartic Archaeon,Methanococcoides burtonii. J. Mol. Biol. 297: 553-567. 2000.
Database WPI Derwent Publications Ltd., London, GB; AN 1996-110275; XP002337559 “Novel yeast promoter active at low temperatures—comprises the Lg-CSP1 promoter region fromS cerevisiae”, & JP 08 009977 A (Kirin Brewery) Jan. 16, 1996 *Abstract*.
Qoronfleh M Walid et al., “Identification and characterization of novel low-temperature-inducible promoters ofEscherichia coli”, Journal of Bacteriology, vol. 174, No. 24, 1992, pp. 7902-7909.
Muiacic M et al., “Cold-inducible cloning vectors for low-temperature protein expression inEscherichia coli: application to the production of a toxic and proteolytically sensitive fusion protein”, GENE, vol. 238, No. 2, 1999, pp. 325-332.
International Search Report of PCT/JP03/05956.
J.C. Varela et al., “TheSaccaromyces cerevisiaeHSP12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A”, Mol. Cell. Biol., 1995, vol. 15, No. 11, pp. 6232-6245.
E. De Groot et al., “Very low amounts of glucose cause repression of the stress-responsive gene HSP12 inSaccharomyces cervisiae”, Microbiology, 2000, vol. 146, No. 2, pp. 367-375.
T. Sahara et al., “Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature”, J. Biol. Chem., Dec. 2002, vol. 277, No. 51, pp. 50015-50021.
L. Zhang et al., “Multiple mechanisms regulate expression of low temperature responsive (LOT) genes inSaccharomyces cerevisiae”, Biochem Biophys Res Commun, 2001, vol. 283, No. 2, pp. 531-535.
Agustin Aranda et al., “Correlation between acetaldehyde and ethanol resistance and expression of HSP genes in yeast strains isolated during the biological aging of sherry wines”, Arch Microbiol (2002) 177: 304-312.
Sonia Rodriguez-Vargas et al., “Gene Expression Analysis of Cold and Freeze Stress in Baker's Yeast”, Applied and Environmental Microbiology, vol. 68, No. 6, Jun. 2002, p. 3024-3030.


Say what you really think

Search for the USA inventors and patents. Rate them and share your experience with other people.


Yeast-origin promoter and vector and expression system using... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Yeast-origin promoter and vector and expression system using..., we encourage you to share that experience with our community. Your opinion is very important and Yeast-origin promoter and vector and expression system using... will most certainly appreciate the feedback.

Rate now


Profile ID: LFUS-PAI-O-3952141

All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.