α-isomaltosylglucosaccharide-forming enzyme, process...

Details α-isomaltosylglucosaccharide-forming enzyme, process... α-isomaltosylglucosaccharide-forming enzyme, process...

2007-05-09

2010-10-12

Fronda, Christian L (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S041000, C435S183000, C435S200000, C536S023200

Reexamination Certificate

active

07811797

ABSTRACT:
The object of the present invention is to provide an α-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an α-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the α-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; α-isomaltosyl-transferring method using the enzyme; method for forming α-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(166)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(16} using both the α-isomaltosylglucosaccharide-forming enzyme and the α-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.

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