Virus detection method and materials

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 7, 435 4, 435805, 435810, 436518, 436523, 436525, 436531, 436532, 436533, 436546, 436800, 436805, 436808, 436810, 436820, 436824, 436825, 422 61, C12Q 170, C12Q 100, G01N 5300, G01N 33543, G01N 33553, G01N 33549, G01N 33546

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046632778

ABSTRACT:
Viruses are detected by means of an immunoassay method in which an extended solid phase coated with antiviral antibody is employed to bind and remove virions from a specimen by forming an immuno-complex with antigens of said virions, a mobile solid phase comprising a dispersion of microspheres coated with the antiviral antibody is used to bind said microspheres to antigens associated with said immuno-complex, and the presence of bound microspheres is detected. The detection sensitivity is amplified by the ability to more readily detect the microspheres, which may be dyed or labelled. The extended solid phase advantageously may be in the form of a dipstick which can be easily contacted with the specimen. A virus detection kit provides the extended solid phase and mobile solid phases, each coated with antiviral antibodies.

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"Diagnostic Virology", Third Ed. (1982), G. D. Hsiung, Yale Univ. Press, New Haven, pp. 3-6.
"Immunology", (1981), The Upjohn Company, Kalamazoo, p. 9.

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