Vector containing viral gene transcribed by RNA polymerase III a

Details Vector containing viral gene transcribed by RNA polymerase III a Vector containing viral gene transcribed by RNA polymerase III a

1996-06-12

1998-11-17

Guzo, David

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 9133, 435 914, 435 9142, 435 9151, 4353201, C12P 1934, C12N 1586

Patent

active

058375031

ABSTRACT:
A recombinant vector containing a cassette for transcription by RNA polymerase III is disclosed. The cassette contains a viral gene transcribed by RNA polymerase III into which an oligonucleotide, a DNA fragment, has been inserted between or outside the boxes A and B constituting the promoter of the viral gene is provided. A method for the intracellular production of an RNA fragment in vitro or in vivo, in which eukaryotic cells containing RNA polymerase III are transfected or infected with a vector according to the invention, containing as oligonucleotides a DNA fragment corresponding to the reverse transcript of the RNA, and in which the eukaryotic cells thus transfected or infected are cultured in a suitable culture medium is also provided. The use of the vectors as a medicinal product is further provided.

REFERENCES:
Miller et al., "Improved Retroviral Vectors for Gene Transfer and Expression", BioTechniques, vol. 7, No. 9, 1989, pp. 980-990.
Stratford-Perricaudet et al., "Gene Transfer into Animals: The Promise of Adenovirus", Human Gene Transfer, vol. 219, 1991, pp. 51-61.
Cotten et al. "Ribozyme Mediated Destruction of RNA in vivo", EMBO J., vol. 8, No. 12, pp. 3861-3866, 1989.
Ghadge et al. "Binding of the Adenovirus VAI RNA to the Interferon-Induced 68-kDa Protein Kinase Correlates with Function", PNAS, vol. 88, pp. 7140-7144, Aug. 1991.
Jennings et al. "Inhibition of SV40 Replicon Function by Engineered Antisense RNA Transcribed by RNA Polymerase III", EMBO J., vol. 6, No. 10, pp. 3043-3047, 1987.

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