Vector comprising DNA sequence coding for enzyme-donor polypepti

Details Vector comprising DNA sequence coding for enzyme-donor polypepti Vector comprising DNA sequence coding for enzyme-donor polypepti

1985-10-22

1992-06-09

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, C12N 1570, C12N 121

Patent

active

051206536

ABSTRACT:
This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

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Casadaban, M. et al., J. Mol. Biol., vol. 138, pp. 179-207, 1980.
Fiddes, J. et al., Nature, vol. 286, pp. 684-687, 1980.
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Miller, F. et al., Gene, vol. 29, pp. 247-250, Jul/Aug, 1984.
Welply, J. et al., J. Biol. Chem., vol. 256, pp. 6804-6810, 1981.
Joachim Messing et al., Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro, Pro. Natl. Acad. Sci., (1977)74:8642-8646.

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