Variants of vascular endothelial cell growth factor

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530350, 530300, C07K 1471

Patent

active

060574283

ABSTRACT:
The present invention involves the preparation of vascular endothelial growth factor (VEGF) variants which provide materials that are selective in respect to binding characteristics to the kinase domain region and the FMS-like tyrosine-kinase region, respectively KDR and FLT-1. The respective KDR and FLT-1 receptors are bound by corresponding domains within the VEGF compound domains. The variants hereof define those two binding regions and modify them so as to introduce changes that interrupt the binding to the respective domain. In this fashion the final biological characteristics of the VEGF molecule are selectively modified.

REFERENCES:
patent: 3691016 (1972-09-01), Patel
patent: 3773919 (1973-11-01), Bowsell
patent: 3887699 (1975-06-01), Yolles
patent: 3969287 (1976-07-01), Jaworek et al.
patent: 4195128 (1980-03-01), Hildebrand et al.
patent: 4229537 (1980-10-01), Hodgins et al.
patent: 4247642 (1981-01-01), Hirohara et al.
patent: 4330440 (1982-05-01), Ayers
patent: 4399216 (1983-08-01), Axel et al.
patent: 5073492 (1991-12-01), Chen et al.
patent: 5219739 (1993-06-01), Tischer et al.
patent: 5332671 (1994-07-01), Ferrara et al.
patent: 5607918 (1997-03-01), Eriksson et al.
Adelman et al., "In Vitro Deletional Mutagenesis for Bacterial Production of the 20,000--Dalton Form of Human Pituitary Growth Horman," DNA, 2(3):183-193 (1983).
Aiello et al., "Vascular endothelial growth factor in ocular fluid of patients with diabetic retinopathy and other retinal disorders," New England J. of Medicine, 331(22):1480-1487 (1994).
Aplin et al., "Preparation, Properties, and Applications of Carbohydrate Conjugates of Proteins and Lipids," CRC Crit. Reb. Biochem., 10(4):259-306 (1981).
Avivi et al., "Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic Acid-Cellulose," Proc. Natl. Acad. Sci. USA, 69:1408-1412 (1972).
Baenziger, "The Oligosaccharides of Plasma Glycoproteins: Synthesis, Structure, and Function," The Plasma Proteins: Structure, Function, and Genetic Control, F.W. Putnam. eds. 2 edition, Academic Press: New York, Chapter 5, vol. IV:271-315 (1984).
Barzu et al., "Binding and endocytosis of heparin by human endothelial cells in culture," Biochim. Biophys. ACTA., 845:196-203 (1985).
Benton et al., "Screening .lambda.gt recombinant clones by hybridization to single plaques in situ," Science, 196(4286):180-182 (1977).
Bolivar et al., "Construction and Characterization of New Cloning Vehicles, II, A Multipurpose Cloning System," Gene, 2:95-113 (1977).
Bothwell et al., Dual expression of .lambda. genes in the MOPC-315 plasmacytoma, Nature, 375:615 (1978).
Chang et al., "Are World Summits Really Necessary," Nature, 290:65-67 (1981).
Chen et al., "High-efficiency transformation of mammilan cells by plasmid DNA," Mol. Cell. Biol., 7(8):2745-2752 (1987).
Cohen et al., "Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia Coliby R-Factor DNA," Proc. Natl. Acad. Sci. USA, 69(8):2110-2114 (1972).
Crea et al., "Chemical Synthesis of Genes for Human Insulin,", Proc. Natl. Acad. Sci. USA, 75(12):5765-5769 (1978).
Creighton, Proteins: Structure and Molecular Properties, W.H. Freeman & Co.: San Francisco. pp. 79-86 (1983).
Ferrara et al., "Pituitary Follicular Cells Secret a Novel Heparin-binding Growth Factor Specific for Vascular Endothelial Cells," Biochem.& Biophys. Res. Comm. 161(2):851-858 (1989).
Ferrara et al, "Vascular endothelial growth factor. The trigger for neovascularization in the eye," Lab. Invest., 72(6):615-618(1995).
Fiers et al., "Complete Nucleotide Sequence of SV40 DNA," Nature, 273:113-120 (1978).
Froehler et al., "Synthesis of DNA via Dio Xynucleoside H-phosphic Intermediates," Nucl. Acids Res., 14(13):5399-5406 (1986).
Gorman et al., "Transient Production of Proteins Using an Adenovirus Transformed Cell Line," DNA and Protein Engineering Techniques, 2(1):3-10 (1990).
Graham et al., "A New Technique for the Assay of Infectivity of Human Adenovirus 5," Virology, 52:456-457 (1973).
Green et al., "Human .beta.globin pre-mRNA sythesized in vitro is accuratley spliced in Xenopus oocyte nuclei," Cell, 32(3):681-694 (1983).
Haniu et al., "Disulfide bonds in recombinant human platelet-derived growth factor BB dimer: characterization of intermolecular and intramolecular disulfide linkages," Biochemistry, 32:2431-2437 (1993).
Hess et al., "Cooperation of Glycolytic Enzymes," Adv. In Enzyme Reg., George Weber, New York: Pergamon Press vol. 7:149-167 (1966).
Hitzeman et al., "Isolation and Characterization of the Yest 3-Phosphoglycerokinase Gene (PGK) by an Immunological Screening Technique," J. Biol. Chem., 255(24):12073-12080 (1980).
Holland et al., "Isolation and Identification of Yeast Messenger Ribonucleic Acids Coding for Enolase, Glyceraldehyde-3-phospate Dehydrogenase, and Phosphoglycerate Kinase," Biochemistry, 17(23):4900-4907 (1978).
Houck et al., "Dual regulation of vascular endothelial growth factor bioavailability by genetic and preteolytic mechanisms," J. Biol. Chem., 267:26031-26037 (1992).
Hsiao et al., "High-frequency Transformation of Yeast by Plasmids Containing the Cloned Yeast Arg4 Gene," Proc. Natl. Acad. Sci. USA, 76:3829-3833 (1979).
Hunter et al., "Preparation of Iodine 131 Labelled Human Growth Hormone of High Specific Activity," Nature, 194:495-496 (1962).
Huynh et al., "Constructing and Screening cDNA Libraries in Agt10 and Agt11," DNA Cloning Techniques, A Practical Approach, (ed. Glover) 1:49-78 (1985).
Itakura et al., "Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin," Science, 198:1056-1068 (1977).
Jones, "Proteinase Mutants of Saccharomyces Cerevisiae," Genetics, 85(1):23-33 (1977).
Kendall et al., "Specificity of Vascular Endothelial Cell Growth Factor Receptor Ligand Binding Domains," Biochem. Biophys. Res., 201(1):326-330 (1994).
Keyt et al., "The Carboxyl-terminal Domain (111-165) of Vascular Endothelial Growth Factor is Critical for its Mitogenic Potency," Journal of Biological Chemistry, 271(13):7788-7795 (1996).
Keyt et al., "Identification of Vascular Endothelial Growth Factor Determinants for Binding KDR and FLT-1 Receptors," Journal of Biological Chemistry, 271(10):5638-5646 (1996).
Kim et al., "The Vascular Endothelial Growth Factor Proteins: Identification of Biologically Relevant Regions by Neutralizing Monoclonal Antibodies," Growth Factors, 7(1):53-64 (1992).
Kingsman et al., "Replication in Saccharomyces Cerevisiae of Plasmid pBR313 Carrying DNA from the Yeast trpl Region," Gene, 7:141-152 (1979).
Kukuruzinska et al., "Protein glycosylation in yeast," Annu. Rev. Biochem., 56:915-944 (1987).
Kunkel et al., "Rapid and Efficient Site-specific Mutagenesis without Phenotypic Slection," Methods Enzymol., 154:367-382 (1987).
Kunkel, "Rapid and Efficient Site-specific Mutagenesis without Phenotypic Slection," Proc. Natl. Acad. Sci., 82:488-492 (1985).
Langer, "Controlled Release of Macromolecules," Chem. Tech., 12:98-105 (1982).
Lawn et al., "The Sequence of Human Serum Albumin cDNA and its Expression in E. Coli", Nucleic Acids Res., 9(22):6103-6114 (1981).
Lusky and Botchen, "Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences," Nature, 293(5827):79-81 (1981).
Maher et al., "Alanine Mutagenesis of Conserved Residues in the Platelet-Derived Growth Factor Family: Identification of Residues Necessary for Dimerization and Transformation," Oncogene, 8:533-541 (1993).
Mandel et al., "Calcium-dependent Bacteriophage DNA Infection," J. Mol. Biol., 53:159-162 (1970).
Maniatis et al., Molecular Cloning: A Laboratory Manual (New York: cold Spring Harbor Laboratory) pp. 31, 90-91, 133-134 (1982).
Maniatis et al., "The Isolation of Structural Gene from Libraries of Eucaryotic DNA," Cell, 15:687-701 (1978).
Marshall, "The nature and metbolism of the carbohydrate-peptide linkage of glycoproteins," Biochem. Soc. Symp., 40:17-26 (1974).
Maxam et al., "Sequencing End-labeled DNA with Bass -Specific Chemical Cleavages," Methods of Enzymology, 65:499-560 (1980).
Messing et al., Proceedings of the Third Clevelan

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Variants of vascular endothelial cell growth factor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Variants of vascular endothelial cell growth factor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Variants of vascular endothelial cell growth factor will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1594847

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.