Variant nucleoside-5′-phosphate producing enzyme

Details Variant nucleoside-5′-phosphate producing enzyme Variant nucleoside-5′-phosphate producing enzyme

2006-01-17

2006-01-17

Nashed, Nashaat T. (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S195000, C435S196000, C435S252330, C435S320100, C536S023100, C536S023200

Reexamination Certificate

active

06987008

ABSTRACT:
There is provided a mutant nucleoside-5′-phosphate producing enzyme with improved nucleoside-5′-phosphate producing ability of a nucleoside-5′-phosphate producing enzyme that has transphosphorylation activity and/or phosphatase activity and has one Lys residue, two Arg residues and two His residues with distances between their Cα's within a particular ranges and a space around them allowing a binding of a nucleoside, of which mutation is designed by using the crystal structure of EB-AP.

REFERENCES:
patent: 6010851 (2000-01-01), Mihara et al.
patent: 6015697 (2000-01-01), Mihara et al.
patent: 6207435 (2001-03-01), Mihara et al.
patent: 0 832 970 (1998-04-01), None
patent: 0 857 788 (1998-08-01), None
patent: WO 96/37603 (1996-11-01), None

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