Variant Hypocrea jecorina CBH1 cellulases

Details Variant Hypocrea jecorina CBH1 cellulases Variant Hypocrea jecorina CBH1 cellulases

2011-07-05

2011-07-05

Desai, Anand U (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S105000, C435S069100, C435S252300, C435S072000, C536S023200, C530S350000

Reexamination Certificate

active

07972832

ABSTRACT:
Described herein are variants ofH. jecorinaCBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.

REFERENCES:
patent: 4435307 (1984-03-01), Barbesgaard et al.
patent: 4822516 (1989-04-01), Suzuki et al.
patent: 5246853 (1993-09-01), Clarkson et al.
patent: 5475101 (1995-12-01), Ward et al.
patent: 5648263 (1997-07-01), Schülein et al.
patent: 5650322 (1997-07-01), Clarkson et al.
patent: 5691178 (1997-11-01), Schülein et al.
patent: 5776757 (1998-07-01), Schülein et al.
patent: 5861271 (1999-01-01), Fowler et al.
patent: 6162782 (2000-12-01), Clarkson et al.
patent: 6255115 (2001-07-01), Beijersbergen et al.
patent: 6562340 (2003-05-01), Bedford et al.
patent: 6599722 (2003-07-01), Boston et al.
patent: 7375197 (2008-05-01), Adney et al.
patent: 2005/0048619 (2005-03-01), Teter et al.
patent: 1368599 (1974-10-01), None
patent: 2094826 (1982-09-01), None
patent: 2095275 (1982-09-01), None
patent: WO 91/04673 (1991-04-01), None
patent: WO 92/06209 (1992-04-01), None
patent: WO 94/28117 (1994-12-01), None
patent: WO 98/21339 (1998-05-01), None
patent: WO 98/31821 (1998-07-01), None
patent: WO 01/04284 (2001-01-01), None
patent: WO 2004/016760 (2004-02-01), None
Chong et al. Nucleic Acids Res. Sep. 25, 1990; 18(18): 5559.
Cheng et al. GenBank Accession No. S11439, created Nov. 21, 1993.
Munoz et al. Family 7 cellobiohydrolases fromPhanerochaete chrysosporium: Crystal structure of the catalytic module of CeI7D (CBH58) at 1.32 A resolution and homology models of the isozymes, J Mol Biol. Dec. 14, 2001;314(5):1097-111.
Altschul, Stephen F. et al., “Basic Local Alignment Search Tool,”J. Mol. Biol., 215:403-410, 1990.
Aro, Nina et al., “ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes ofTrichoderma reesei,” J. Biol. Chem., vol. 276, No. 26, pp. 24309-24314, Jun. 29, 2001.
Aubert, et al., Ed., p11 et seq., Academic Press, 1988.
Ausubel, et al., Current Protocols in Molecular Biology, Greene Publishing, Associates and Wiley Interscience, N.Y. (1994).
Bajar, Aslam, et al., “Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor,”Proc. Natl. Acad. Sci. USA, vol. 88, pp. 8208-8212, Sep. 1991.
Ballance, D.J., et al., “Transformation ofAspergillus nidulansby the Orotidine-5′-Phosphate Decarboxylase Gene ofNeurospora crassa,” Biochemical and Biophysical Research Communications, vol. 112, No. 1, pp. 284-289, Apr. 15, 1983.
Baulcombe, D., “Viruses and gene silencing in plants,”Archives of Virology, Calisher and Horzinek eds., Springer-Verlag, New York, NY 15:189-201, 1999.
Becker, Dieter, et al., “Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour ofTrichoderma reeseiCeI7A and its E223S/A224H/L225V/T226A/D262G mutant,”Biochem. J., 356:19-30 (2001).
Berges, Thierry et al., “Isolation of uridine auxotrophs fromTrichoderma reeseiand efficient transformation with the clonedura3andura5genes,”Curr. Genet.vol. 19, pp. 359-365, 1991.
Bhikhabhai, Ramagauri et al., “Isolation of Cellulolytic Enzymes fromTrichoderma reesei QM 9414,” J. Appl. Biochem. 6:336-345, 1984.
Brumbauer, Aniko et al., “Fractionation of cellulase and β-glucosidase in aTrichoderma reeseiculture liquid by use of two-phase partitioning,” Bioseparation 7:287-295, 1999.
Burley, S.K. et al., “Aromatic-Aromatic Interaction: A Mechanism of Protein Structure Stabilization,”Science229:23-28, 1985.
Cadwell, Craig R., et al., “Randominzation of Genes by PCR Mutagenesis,”PCR Methods and Applications, 2:28-33, 1992.
Campbell, Edward I., et al., “Improved transformation efficiency ofAspergillus nigerusing the homologousniaDgene for nitrate reductase,” Current Genetics, 16:53-56, 1989.
Cao, Qing-Na et al., “Penicillopepsin-JT2, a recombinant enzyme fromPenicillium janthinellumand the contribution of a hydrogen bond in subsite S3to kcat,” Protein Science, vol. 9, pp. 991-1001, 2000.
Carter, Paul et al., “Improved oligonucleotide site-directed mutagenesis using M13 vectors,”Nucleic Acids Research, vol. 13, No. 12, pp. 4431-4443, 1985.
Cees, A. M. et al., “Heterologous Gene Expression in Filamentous Fungi,”More Gene Manipulations in Fungi, Bennett, J.W. et al., ed., pp. 396-428, Academic Press, 1991.
Collgan, J. E. et al., eds., Current Protocols in Immunology, 1991.
Coughlan, Michael, P. et al., “Comparative Biochemistry of Fungal and Bacterial Cellulolytic Enzyme Systems,”Biochemistry and Genetics of Cellulose Degradation, pp. 11-30 1988.
Cummings, C. et al., “Secretion ofTrichoderma reeseiβ-glucosidase bySaccharomyces cerevisiae,” Curr. Genet. 29:227-233, 1996.
Davies, Gideon J., “Oligosaccharide specificity of a family 7 endoglucanase: Insertion of potential sugar-binding subsites,”Journal of Biotechnology, 57:91-100, 1997.
Deutscher, Murray P., “Rethinking Your Purification Procedure,” Methods in Enzymology, vol. 182, No. 57, p. 779, 1990.
Divne, Christina, et al., “High-resolution Crystal Structures Reveal How a Cellulose Chain is Bound in the 50 Å Long Tunnel of Cellobiohydrolase I fromTrichoderma reesei,” J. Mol. Biol., 275:309-325, 1998.
Ellouz, S. et al., “Analytical Separation ofTrichoderma reeseiCellulases by Ion-Exchange Fast Protein Liquid Chromatography,”Journal of Chromatography, 396:307-317, 1987.
Eriksson, A. E., et al., “Response of a Protein Structure to Cavity-Creating Mutations and its Relation to the Hydrophobic Effect,”Science, 255:178-183, 1992.
Filho, Edivaldo X. F., “Purification and characterization of a β-glucosidase from solid-state cultures ofHumicola griseavar.thermoidea,” Can. J. Microbiol., 42:1-5, 1996.
Fliess, A., et al., “Characterization of Cellulases by HPLC Separation,” Eur. J. Appl. Microbiol. Biotechnol., vol. 17, pp. 314-318, 1983.
Freer, Shelby N., “Kinetic Characterization of a β-Glucosidase from a Yeast,Candida wickerhamii,” J. Biol. Chem., vol. 268, No. 13, pp. 9337-9342, 1993.
Freshney, R. I., ed., Animal Cell Culture, 1987.
Gloss, Lisa M., et al., “Urea and Thermal Equilibrium Denaturation Studies on the Dimerization Domain ofEscherichia coil TrpRepressor,”Biochem., vol. 36, No. 19, pp. 5612-5623, 1997.
Goedegebuur, Frits, et al., “Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase,” Current Genetics, vol. 41, pp. 89-98, 2002.
Goldman, G. H., et al., “Transformation ofTrichoderma harzianumby high-voltage electric pulse,”Current Genetics, 17:169-174, 1990.
Goyal, Anil, et al., “Characteristics of Fungal Cellulases,”Bioresource Technology, vol. 36, pp. 37-50, 1991.
Hale & Markham, The Harper Collins Dictionary of Biology, Harper Perennial, NY, 1991.
Halldorsdottir, S, et al., “Cloning, sequencing and overexpression of aRhodothermus marinusgene encoding a thermostable cellulase of glycosyl hydrolase family 12,”Appl. Microbiol. Biotechnol., 49(3):277-284, 1998.
Hemmpel, W. H., “The surface modification of woven and knitted cellulose fibre fabrics by enzymatic degradation,”ITB Dyeing/Printing/Finishing, 3:5-14, 1991.
Henikoff, Steven, et al., “Amino acid substitution matrices from protein blocks,”Proc. Natl. Acad. Sci. USA, 89:10915-10919, Nov. 1992.
Higuchi, Russell, “Recombinant PCR,”PCR Protocols: A Guide to Methods and Applications, pp. 177-183, Academic Press, Inc. 1990.
Himmel, Michael E., “Adanced Bioethanol Production Technologies: A Perspective,”American Chemical Society, pp. 2-45, 1997.
Hopwood, et al., “Genetic Manipulation of Streptomyces: A Laboratory Manual,” Innes, 1985.

Affiliated with

Also associated with

Rating

Variant Hypocrea jecorina CBH1 cellulases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Variant Hypocrea jecorina CBH1 cellulases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Variant Hypocrea jecorina CBH1 cellulases will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2650560