Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-07
1998-04-21
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 435220, 435221, 4352523, 43525231, 4353201, 4351723, 536 232, 510300, C12N 956, C12N 950, C12N 954, C11D 3386
Patent
active
057416940
ABSTRACT:
The present invention relates to mutations of the subtilisin gene, some of which result in changes in the chemical characteristics of subtilisin enzyme. Mutations are created at specific nucleic acids of the subtilisin gene and, in various specific embodiments, the mutant enzymes possess altered chemical properties including, but not limited to, increased stability to oxidation, augmented proteolytic activity, and improved washability. The present invention also relates, but is not limited to, the amino acid and DNA sequences for two proteases derived from Bacillus lentus variants, subtilisin 147 and subtilisin 309, as well as mutations of these genes and the corresponding mutant enzymes.
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Betz
Aaslyng Dorrit
Branner Sven
Hastrup Sven
Jensen Villy Johannes
N.o slashed.rskov-Lauridsen Leif
Lambiris Elias J.
Moore William W.
Novo Nordisk A S
Wax Robert A.
Zelson Steve T.
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