Use of viral CIS-acting post-transcriptional regulatory sequence

Details Use of viral CIS-acting post-transcriptional regulatory sequence Use of viral CIS-acting post-transcriptional regulatory sequence

1996-03-11

1998-04-28

Guzo, David

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 536 241, C12N 1563, C12N 1579, C07H 2104

Patent

active

057443262

ABSTRACT:
Expression vectors are disclosed comprising intronless genes containing one or more near consensus splice sequences and one or more copies of a viral cis-acting post-transcriptional regulatory element which is transcribed along with the gene and causes export of the gene transcript from the nucleus into the cytoplasm of the cell. In a preferred embodiment, the vectors are targeted for delivery to specific cells in the form of a molecular complex made up of the plasmid releasably linked to a nucleic acid binding agent and a ligand which binds to a component on the surface of a cell. Use of viral cis-acting post-transcriptional regulatory elements as disclosed can increase expression of intronless genes with near-consensus splice sites.

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