Use of the repressor glxR for the synthesis of lysine in...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S252320, C435S069100, C435S320100, C435S193000, C536S023200

Reexamination Certificate

active

10514076

ABSTRACT:
Isolated polypeptide sequence having the sequence of SEQ ID NO:1 or muteins thereof having the ability to bind cAMP and repress the expression of the aceB gene ofC. glutamicumand which can be obtained from SEQ ID NO:1 by inserting, deleting or substituting up to 20% of the amino acids.

REFERENCES:
patent: 1 108 790 (2001-06-01), None
patent: 1 362 866 (2003-11-01), None
Chung T, et al. Glyoxylate bypass operon ofEscherichia coli: cloning and determination of the functional map. J Bacteriol. Jan. 1988;170(1):386-92.
Database Accession No. AX123710. Novel Polypeptides. May 10, 2001.
Database Accession No. BD571237. Novel Polynucleotides. Jan. 17, 2003.
Database Accession No. AF293334. Probable Transcription Regulator. Jun. 8, 2001.
Database Accession No. Q8FTU1. The entire genomic sequence ofCorynebacterium efficiensYS-314. Mar. 1, 2003.
Database Accession No. AB083047. drp86 ofCorynebacterium glutamicum.Apr. 3, 2002.
Database Accession No. AAG90072. Sep. 26, 2001.
Kim, et al. Utilization of LACZ to isolate regulatory genes fromCorynebacterium glutamicum.Journal of Microbiology & Biotechnology. Apr. 2002. 12(2):336-339.
Lee, et al. Molecular Characterization of AceB, a gene encoding malate synthase inCorynebacterium glutamicum.Journal of Microbiology & Biotechnology. 1994 4(4):256-263.
Reinscheid, et al. Characterization of the isocitrate lyase gene fromCorynebacterium glutamicumand biochemical analysis of the enzyme. J Bacteriol. Jun. 1994;176(12):3474-83.
Sunnarborg, et al. Regulation of the glyoxylate bypass operon: cloning and characterization of iclR. J Bacteriol. May 1990;172(5):2642-9.
Wendisch VF, et al. Regulation of acetate metabolism inCorynebacterium glutamicum:transcriptional control of the isocitrate lyase and malate synthase genes. Arch Microbiol. Oct. 1997;168(4):262-9.
International Search Report for PCT/EP03/04969 Mailed Jan. 24, 2004.

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