Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-05-17
1997-01-07
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 72, 435193, 435195, 435200, 435201, 53612313, C12P 1912, C12N 926, C12N 914, C12N 910
Patent
active
055916120
ABSTRACT:
Disclosed is a trehalose-releasing enzyme which specifically hydrolyzes the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. The molecular weight of the enzyme is about 57,000 to 68,000 daltons on SDS-PAGE, and the isoelectric point is about 3.3 to 4.6 on isoelectrophoresis. The enzyme is useful in an industrial-scale preparation of trehalose, and the trehalose prepared therewith can be readily incorporated into food products, as well as cosmetic- and pharmaceutical-compositions.
REFERENCES:
patent: 4762857 (1988-08-01), Bollin, Jr. et al.
patent: 5169767 (1992-12-01), Matsuura et al.
patent: 5218096 (1993-06-01), Shibuya et al.
Kubota Michio
Maruta Kazuhiko
Miyake Toshio
Sugimoto Toshiyuki
Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
Prats Francisco C.
Wityshyn Michael G.
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