Trehalose phosphorylase, its preparation and use

Details Trehalose phosphorylase, its preparation and use Trehalose phosphorylase, its preparation and use

1998-12-22

1999-11-30

Lilling, Herbert J.

Food or edible material: processes, compositions, and products

Products per se, or processes of preparing or treating...

Carbohydrate containing

424 7013, 426 48, 426 61, 426 63, 426103, 435 691, 435 98, 435 99, 435100, 435101, 435 74, 435193, 4352523, 4353201, 536 41, 536123, 5361231, 53612313, 536124, 536 232, 536 237, A61K 700, A23L 109, A23L 128, A23G 300, C12N 910

Patent

active

059938897

ABSTRACT:
A thermostable trehalose phosphorylase which is obtainable from microorganisms of the genus Thermoanaerobium and which hydrolyzes trehalose in the presence of an inorganic phosphoric acid to form D-glucose and .beta.-D-glucose-1-phosphoric acid. The trehalose phosphorylase can be also prepared by recombinant DNA technology. When the enzyme is allowed to contact with .beta.-D-glucose-1-phosphoric acid as a saccharide donor in the presence of other saccharides, glucosyl-transferred saccharides including glucosyl-D-galactoside, which are conventionally known but scarcely obtainable, can be produced on an industrial-scale and in a relatively-low cost.

REFERENCES:
patent: 4628028 (1986-12-01), Katkocin et al.
patent: 5643775 (1997-07-01), Takahashi et al.
Sigma Catalogue p. 969 Trehalose T8778, 1992.
Gherna et al (Eds), "Cataloge of Bacteria and Bacteriophages", 18th Ed., American Type culture Collection (Rockville, MD), pp. v, 366 (1992).
Kizawa et al, "Purification and Characterization of Trehalose Phosphorylase from Micrococcus varians", Biosci. Biotech. Biochem. 59(10):1908-1912 (1995).
Murao et al, "Enzymatic Synthesis of Trehalose from Maltose", Agric. Biol. Chem. 49(7):2113-2118 (1985).
Marechal et al, "Metabolism of Trehalose in Euglena gracilis", J. Biol. Chem. 247(10):3223-3228 (1972).
Sambrook et al, Molecular Cloning: A Laboratory Manual, 2nd. Ed., Cold Spring Harbor Laboratory Press (Cold Spring Harbor, NY, 1989).
Yoshida et al, "Production and Application of Maltose Phosphorylase and Trehalose Phosphorylase by a Strain of Plesiomonas", Oyo Tashitsu Kagaku 42(1):19-25 (1995).

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