Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Reexamination Certificate
2011-08-02
2011-08-02
Barton, Jeffrey T (Department: 1759)
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
C204S643000
Reexamination Certificate
active
07988841
ABSTRACT:
A plurality of planar electrodes (5) in a microchannel (4) is used for separation, lysis and PCR in a chip (10). Cells from a sample are brought to the electrodes (5). Depending on sample properties, phase pattern, frequency and voltage of the electrodes and flow velocity are chosen to trap target cells (16) using DEP, whereas the majority of unwanted cells (17) flushes through. After separation the target cell (16) are lysed while still trapped. Lysis is carried out by applying RF pulses and/or thermally so as to change the dielectric properties of the trapped cells. After lysis, the target cells (16) are amplified within the microchannel (4), so as to obtain separation, lysis and PCR on same chip (1).
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Mueller Torsten
Schnelle Thomas
Scurati Mario
Ball J. Christopher
Barton Jeffrey T
Boulware & Valoir
STMicroelectronics S.r.l.
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