Multicellular living organisms and unmodified parts thereof and – Nonhuman animal
Patent
1995-04-07
1998-10-06
Chambers, Jasemine C.
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
4351723, 435 691, 424 91, C12N 1563, C12N 1500, A61K 4800
Patent
active
058179119
ABSTRACT:
A transgenic, non-human animal is provided which overexpresses a gene responsible for the accumulation of monocytes and leukocytes. The animal is preferably a mammal and more preferably a mouse, rat or guinea pig. The transgenic animal is created by artificially inserting a transgene into a fertilized egg of the animal which egg is then inserted within a pseudo pregnant female where it is allowed to grow. The transgene preferably expresses human Monocyte Chemoattractant Protein-1 (MCP-1) and more preferably overexpresses MCP-1 in type II pulmonary epithelial cells. The invention includes DNA constructs and vectors containing the constructs with a particularly preferred vector being SPC-MCP-1. The plasmid SPC-MCP-1 includes a promoter operatively linked to a human MCP-1 coding sequence. The transgenic animal provides a useful animal model for testing drugs for their efficacy with respect to the treatment of diseases and conditions which result in an acceptable high accumulation of monocytes and/or lymphocytes.
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Lee et al.,
Gunn Michael Dee
Williams Lewis T.
Chambers Jasemine C.
Hauda Karen M.
Regents of the University of California
Sherwood Pamela J.
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