Transformant producing secondary metabolite modified with...

Details Transformant producing secondary metabolite modified with... Transformant producing secondary metabolite modified with...

2006-09-19

2006-09-19

Kam, Chih-Minh (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S320100, C536S023100, C530S350000

Reexamination Certificate

active

07109018

ABSTRACT:
An objective of the present invention is to provide a transformant altered so as to produce a secondary metabolite in which a benzene ring of the secondary product is modified at the para-position with a functional group containing a nitrogen atom. A transformant according to the present invention is a transformant of an organism producing a secondary metabolite having a benzene ring skeleton without substitution at the para-position with a functional group containing a nitrogen atom, said transformant being transformed by introducing genes involved in a biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid, including a gene encoding an amino acid sequence (SEQ ID NO: 2) having 4-amino-4-deoxychorismic acid synthase activity, a gene encoding an amino acid sequence (SEQ ID NO: 4) having 4-amino-4-deoxychorismic acid mutase activity and a gene encoding an amino acid sequence (SEQ ID NO: 6) having 4-amino-4-deoxyprephenic acid dehydrogenase activity, so as to produce a secondary metabolite having a benzene ring skeleton substituted at the para-position with a functional group containing a nitrogen atom. Another objective of the present invention is to provide a novel gene involved in the biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid. A novel gene according to the present invention comprises genes encoding the amino acid sequences of SEQ ID NOs: 2, 4 and 6 or modified sequences thereof.

REFERENCES:
patent: 2990325 (1961-06-01), Donovick et al.
patent: 5763221 (1998-06-01), Aoyagi et al.
patent: 2002/0072062 (2002-06-01), Paradkar et al.
patent: 0 634 408 (1995-01-01), None
patent: 0 685 469 (1995-12-01), None
patent: 0 903 347 (1996-09-01), None
patent: 0 780 468 (1997-06-01), None
patent: 0 930 304 (1999-07-01), None
patent: 96/01901 (1996-01-01), None
patent: 97/00944 (1997-01-01), None
patent: 97/11064 (1997-03-01), None
patent: 97/20945 (1997-06-01), None
GenBank Accession No. U21728 (1996)Streptomyces venezuelaep-aminobenzoic acid synthase (pabAB) gene.
GenBank Accession No. AAB30312 (1996) p-aminobenzoic acid synthase [Streptomyces venezuelae].
He et al. The gene clsuter for chloramphenicol biosynthesis inStreptomyces venezuelaeISP5230 includes novel shikimate pathway homolgues and a monomodular non-ribosomal peptide synthetase gene. Microbiology (2001) 147: 2817-2829.
GenBank Accession No. AF262220 (Oct., 2000)Streptomyces venezuelaechloramphenicol biosynthetic gene cluster, partial sequence.
Miyadoh et al. Taxonomic position of the fungus producing the anthelmintic PF1022 based on the 18S rRNA gene base sequence. Nippon Kingakkai Kaiho (2000) 41(4): 183-188 (Abstract only).
Yanai et al. Para-position derivatives of fungal anthelmintic cyclodepsipeptides engineered withStreptomyces venezuelaeantibiotic biosynthetic genes. Nature Biotechnology (2004) 22(7): 848-855.
Michael P. Brown et al., “A role for pabAB, a p-aminobenzoate synthase gene ofStreptomyces venezuelaeISP5230, in chloramphenicol biosynthesis”, Microbiology 142, pp. 1345-1355 (1996).
V. Blanc et al., “Identification and analysis of gened fromStreptomyces pristinaespiralisencoding enzymes involved in the biosynthesis of the 4-dimethylamino-L-phenylalanine precursor of pristinamycin I”, Molecular Microbiology, vol. 23, No. 2, pp. 191-202, 1997.
J. Doull et al., “Isolation and Characterization ofStreptomyces venezuelaeMutants Blocked in Chloramphenicol Biosynthesis” Journal of General Microbiology, vol. 131, pp. 97-104, 1985.
M.P. Brown et al., “A role for pabAB, a p-aminobenzoate synthase gene ofStreptomyces venezuelaeISP5230, in chloramphenicol biosynthesis” ,Microbiol.,vol. 142, pp. 1345-1355, (1996).
B.P. Nichols et al., “para-Aminobenzoate Synthesis from Chorismate Occurs in Two Steps”,Journal of Biological Chemistry, vol. 264, No. 15, pp. 8597-8601, (1989).

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