Transducing particles and methods for their production

Details Transducing particles and methods for their production Transducing particles and methods for their production

1990-11-05

1993-02-16

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

4351723, 4352351, 4352523, 43525233, C12N 1500, C12N 701, C12N 121

Patent

active

051870614

ABSTRACT:
Viable bacteria may be detected in biological samples by exposing bacterial cultures obtained from the samples to transducing particles having a known host range. Such transducing particles carry a heterologous gene capable of altering the phenotype of the bacteria in a readily detectable manner. For example, the transducing particles may carry an ice nucleation gene and the alteration of phenotype may be detected using an ice nucleation assay. By employing a panel of phage, unknown bacteria nmay be typed based on the pattern of reactivity observed. The transducing particles may be prepared by introducing a synthetic transposable element carrying the heterologous gene to a host carrying a prophage having the desired host range. After transposition, the host may be induced to a lytic cycle to release the transducing particles carrying the heterologous gene.

REFERENCES:
patent: 4464473 (1984-08-01), Orser et al.
patent: 4689295 (1987-08-01), Taber et al.
patent: 4861709 (1989-08-01), Ulitzur et al.
Dodson et al. (Mar. 30, 1989), Gene 76, pp. 207-213.
Makela, Enterobacterial Surface Antigens: Methods for Molecular Characterization, Korhonen et al., eds. Elsevier Science Publishing, Amsterdam, pp. 155-178 (1985).
Susskind et al. (1978) Microbiol. Rev. 42:385-413.
Watanabe et al. (1972) Virol. 50:874-882.
Orbach and Jackson (1982) J. Bacteriol. 149:985-994.
Schmidt and Schmieger (1984) Mol. Gen. Genet. 196:123-128.
Vogel and Schmieger (1986) Mol. Gen. Genet. 205:563-567.
Spanova and Karlovsky (1986) Folia Microbiol. 31:353-363.
Young et al. (1983) Proc. Natl. Acad. Sci. USA 80:1194-1198.
Viera et al. (1987) Meths. Enz. 153:3-11.
ASM News (1987) 53:542.
Orser et al. Molecular Genetics of the Bacterial-Plant Interaction (A. Puhler, ed.), Elsevier/North Holland Biomedical, pp. 353-361 (1983).
Green et al. (1985) Nature 317:645-648.
Corotto et al. (1986) EMBO J. 5:231-236.
Warren et al. (1986) Nuc. Acids. Res. 14:8047-8060.
Wolber et al. (1986) Proc. Natl. Acad. Sci. USA 83:7256-7260.
Vali (1971) J. Atmos. Sci. 28:402-409.

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