Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1994-04-18
1996-05-07
Jordan, Charles T.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C12N 912
Patent
active
H00015318
ABSTRACT:
The invention relates to a substantially pure thermostable DNA polymerase. Preferably, the DNA polymerase has a molecular weight of about 95 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to cloning and expression of the DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.
REFERENCES:
patent: 4795699 (1989-01-01), Tabor et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5047342 (1991-09-01), Chatterjee
Bergquist et al., "Genetics and Potential Biotechnological Applications of Thermophilic and Extremely Thermophilic Micro-organisms," Biotech. & Genet. Eng. Rev. 5:199-244 (Sep. 1987).
Blanco et al., "Evidence favouring the hypothesis of a conserved 3'-5' exonuclease active site in DNA-dependent DNA polymerases," Gene 112:139-144 (1992).
Bragger et al., "Very stable enzymes from extremely thermophilic archaebacteria and eubacteria," Appl. Microbiol. Biotechnol. 31:556-561 (1989).
Braithwaite & Ito, "Compilation, alignment, and phylogenetic relationships of DNA polymerases," Nucl. Acids Res. 21:787-802 (1993).
Chien et al., "Deoxyribonucleic Acid Polymerase from the Extreme Thermophile Thermus aquaticus," J. Bacteriol. 127:1550-1557 (Sep. 1976).
Cowan et al., "An extremely thermostable extracellular proteinase from a strain of the archaebacterium Desulfurococcus growing at 88.degree. C.," J. Biochem. 274:121-133 (1987).
Elie et al., "Thermostable DNA polymerase from the archaebacterium Sulfolobus acidocaldarius: Purification, characterization and immunological properties," Eur. J. Biochem. 178:619-626 (1989).
Gelfand & White, "Thermostable DNA Polymerases," in: PCR Protocols: A Guide to Methods and Applications, Innis et al. (eds.) Academic Press, Inc., San Diego, CA, pp. 129-130 (1990).
Kaboev et al., "Purification and Properties of Deoxyribonucleic Acid Polymerase from Bacillus stearothermophilus," J. Bacteriol. 145:21-26 (Jan. 1981).
Kaledin et al., "Isolation and Properties of DNA Polymerase from Extremely Thermophilic Bacterium Thermus aquaticus YT1," Biokhimiya 45:644-651 (Apr. 1980).
Kelly & Deming, "Extremely Thermophilic Archaebacteria: Biological and Engineering Considerations," Biotech. Prog. 4:47-62 (Jun. 1988).
Klimczak et al., "Purification and Characterization of DNA Polymerase from the Archaebacteria Methanobacterium thermoautotrophicum," Biochem. 25:4850-4855 (1986).
Lin et al., "Cloning and expression of T4 DNA polymerase," Proc. Natl. Acad. Sci. USA 84:7000-7004 (Oct. 1987).
Mattila et al., "Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity," Nucl. Acids Res. 19:4967-4973 (Sep. 25, 1991).
Minkley et al., "Escherichia coli DNA Polymerase I," J. Biol. Chem. 259:10386-10392 (Aug. 25, 1984).
Patel et al., "Isolation of anaerobic, extremely thermophilic, sulphur metabolising archaebacteria from New Zealand hot springs," New Zealand J. Marine & Freshwater Res. 20:439-445 (1986).
Reddy et al., "Hyperexpression and purification of Escherichia coli adenylate cyclase using a vector designed for expression of lethal gene products," Nucl. Acids Res. 17:10473-10488 (1989).
Rossi et al., "Structure and Properties of a Thermophilic and Thermostable DNA Polymerase Isolated from Sulfolobus solfataricus," System. Appl. Microbiol. 7:337-341 (1986).
Simpson et al., "Purification and some properties of a thermostable DNA polymerase from a Thermotoga species," Biochem. Cell Biol. 68:1292-1296 (1990).
Stenesh & Roe, "DNA Polymerases from Mesophilic and Thermophilic Bacteria: Purification and Properties of DNA Polymerase from Bacillus licheniformis and Bacillus stearothermophilus," Biochim. Biophys. Acta 272:156-166 (19872).
Blumentals Ilse I.
Chatterjee Deb K.
Hughes, Jr. A. John
Lasken Roger S.
Longo Mary C.
Hardee John R.
Jordan Charles T.
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