T-DNA promoters

Details T-DNA promoters T-DNA promoters

1985-04-22

1993-01-26

Fox, David T.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 435 701, 4352404, 4352522, 4352523, 43525233, 536 241, 536 232, 536 236, 935 35, 935 36, 935 41, 935 67, C12N 1500, C12N 500, C12R 141, C12P 2100, C07H 1512

Patent

active

051822008

ABSTRACT:
The sequence of the T.sub.L -DNA of Ri plasmids found in Agrobacterium rhizogenes strains HRI and A4 is disclosed. Sixteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found, five of which were observed to be transcripted in a developmentally and phenotypically regulated manner. The use of promoters and polyadenylation sites from pRi T.sub.L -DNA to control expression of heterologous foreign structural genes is taught, using as examples the structural genes for Phaseolus vulgaris storage protein (phaseolin), P. vulgaris lectin, a sweet protein (thaumatin), and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

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