Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters ethylene production in the plant
Patent
1998-09-18
2000-09-12
Nelson, Amy J.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters ethylene production in the plant
4353201, 435419, 536 241, C07H 2104, C12N 514, C12N 1582
Patent
active
061180491
ABSTRACT:
The present invention is directed to a synthetic hybrid promoter composed of polynucleotide segments derived from the E8 and E4 gene promoters. The hybrid promoter is capable of providing high-level expression of heterologous genes, particularly in transformed fruit. DNA constructs containing the E8-E4 hybrid promoter operably linked to an exemplary heterologous SAMase gene are effective in conferring a delayed ripening phenotype to transformed fruit.
REFERENCES:
patent: 5416250 (1995-05-01), Ferro et al.
patent: 5512466 (1996-04-01), Klee et al.
patent: 5723746 (1998-03-01), Bestwick et al.
patent: 5750864 (1998-05-01), Bestwick et al.
Kay R, et al. "Duplication of CaMV 35S promoter sequences." Science 236: 1299-1302, Jun. 1987.
Benfey PN, et al. "The cauliflower mosaic virus 35S promoter: Combinatorial regulation of transcription in plants." Science 250: 959-966, Nov. 1990.
Kim Y, et al. "A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity." Plant Mol. Biol. 24: 105-117 1994.
Cordes, S., et al., "Interaction of a Developmentally Regulated DNA-Binding Factor with Sites Flanking Two Different Fruit-Ripening Genes from Tomato," The Plant Cell 1:1025-1034 (1989).
Deikman, J., et al., "Control of Gene Transcription by Ethylene During Tomato Fruit Ripening," Biology and Biotechnology of the Plant Hormone Ethylene, A.K. Kanellis et al (eds.), Kluwer Acadmic Publishers, pp. 123-131, 1997.
Deikman, J., et al., "Interaction of a DNA binding factor with the 5'-flanking region of an ethylene-responsive fruit ripening gene from tomato," The EMBO Journal 7(11): 3315-3320 (1988).
Deikman, J., et al., "Organization of Ripening and Ethylene Regulatory Regions in a Fruit-Specific Promotor from Tomato (Lycopersicon esculentum)," Plant Physiol. 100:2013-2017 (1992).
Genbank Accession No. S44898 E4=fruit ripening gene, 1992.
Genbank Accession No. X13437 Tomato ethylene-responsive fruit ripening gene E8, 1995.
Montgomery, Julie, et al., "Identification of an ethylene-responsive region in the promoter of a fruit ripening gene," Proc. Natl. Acad. Sci USA 90:5939-5943 (1993).
Valles, M.P., et al, "Agrobacterium-mediated transformation of commercial melon (Cucumis melo L., cv. Amarillo Oro," Plant Cell Reports 13:145-148 (1994).
Xu, R., et al., "Ethylene control of E4 transcription during tomato fruit ripening involves two cooperative cis elements," Plant Molecular Biology 31: 1117-1127 (1996).
Bestwick Richard K.
Kellogg Jill Anne
Agritope, Inc.
Judge Linda R.
Nelson Amy J.
LandOfFree
Synthetic hybrid tomato E4/E8 plant promoter does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Synthetic hybrid tomato E4/E8 plant promoter, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthetic hybrid tomato E4/E8 plant promoter will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-97814