Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1990-05-21
1994-07-19
Elliott, George C.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
43524023, 530329, 530330, C12N 506, C07K 510, C07K 706
Patent
active
053309111
ABSTRACT:
An absorbed protein-independent cell-adhesive surface is disclosed. The treated surface comprises a chemically derivatized material to which small peptides, having less than 12 amino acid residues and including YIGSR, RGD or REDV amino acid sequence are covalently linked to. The peptides of the present invention include a terminal glycine amino acid. Tresyl chloride activation of surface hydroxyl moieties provides the active surface sites by which a terminal glycine arm of a selected peptide attaches to form covalent bonds between the substrate and peptide. Peptides high with cell adhesive properties are bound in high efficiency.
By way of example, surfaces which may be used in conjunction with the present invention include polymer, metal, and ceramic surfaces. The most preferred polymer surfaces include PHEMA and PET polymer surfaces, with the most preferred glass surfaces being glycophase glass.
The present methods also include a pretreatment method which provides hydroxyl moieties to surfaces devoid of readily available hydroxyl moieties. The pretreatment, by way of example, comprises immersion of the surface in a mixture of formaldehyde and acetic acid.
Methods of preparing the treated surfaces are also included in the present invention.
Also included are surface-treated biomedical implant devices and cell culturing devices. The treated surface promotes an enhanced rate and an enhanced amount of cell adhesion to the surface, independent of media serum concentrations or other absorbed proteins. The treated surfaces of the present invention are thermally stable, reusable, peptide efficient (attached to surface only) and resistant to cell proteolysis.
The invention further concerns polymeric substrates with a surface having physically interpenetrating water-soluble polymer chains, and methods for production thereof.
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USA Ser. No. 278,781 Aug. 17, 1988.
Desai Neil P.
Hubbell Jeffrey A.
Massia Stephen P.
Board of Regents , The University of Texas System
Elliott George C.
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