Subtilisin mutants lacking a primary calcium binding site

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435220, 435221, 4351723, 435 691, 4352523, 43525231, 4353201, 536 232, 935 14, 935 29, 935 74, C12N 956, C12N 1511, C12N 1557, C12N 1575

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055676014

ABSTRACT:
Novel calcium free subtilisin mutants are taught, in particular subtilisins which have been mutated to eliminate amino acids 75-83 and which retain enzymatic activity and stability. Recombinant methods for producing same and recombinant DNA encoding for such subtilisin mutants are also provided.

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