Streptococcus Lactis containing plasmids encoding for mucoidness

Details Streptococcus Lactis containing plasmids encoding for mucoidness Streptococcus Lactis containing plasmids encoding for mucoidness

1988-05-02

1991-11-19

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 435 711, 435 91, 435170, 4351721, 4352523, 4353201, 435885, 536 27, 935 6, 935 9, 935 22, 935 29, 935 59, 935 72, C12N 121, C12N 1510

Patent

active

050665880

ABSTRACT:
A lactose-fermenting (Lac.sup.+), mucoid (Muc.sup.+) Streptococcus lactis transconjugant designated ML-3/2.230 was obtained by conjugative co-transfer of Lac.sup.+ Muc.sup.+ phenotypes from Lac.sup.+ Muc.sup.+ Streptococcus cremoris MS to lactose-negative (Lac.sup.+), non-mucoid S. lactis ML-3/2.2. Curing studies on the transconjugant ML-3/2.230 showed that Muc.sup.+ phenotype in the strain was encoded on two plasmids, pSRQ2203 and pSRQ2204. DNA hydridization experiments also indicated the involvement of two plasmids in the expression of the mucoid phenotype. Plasmid pSRQ2203 also contained genetic information for lactose utilization (Lac.sup.+).

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