Stable integration of DNA in bacterial genomes

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 691, 4353201, C12N 1564, C12N 1565, C12N 1575, C12P 2102

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056959761

ABSTRACT:
A bacterial cell which in its genome carries an integrated non-replicative DNA construct comprising (1) a DNA sequence of interest, (2) a DNA sequence which is homologous with a region of the genome of the cell, and (3) an origin of replication, the DNA construct lacking a functional gene coding for a factor required to initiate replication from the origin of replication.

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