Stable hemoglobin reference solution

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435 11, 435 13, 435 15, 435 16, 435 18, 435 25, 436 11, 436 15, C12Q 100, C12Q 160, C12Q 156, G01N 3100

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055589855

ABSTRACT:
A reference solution for calibrating and monitoring blood gas instrumentation that has a shelf line of two years or greater is disclosed. The solution is contained in a closed container having a gas or gas mixture that is inert to the reference solution and comprises an aqueous mixture containing a hemoglobin, a source of bicarbonate ions and an organic buffer. The solution optionally contains a methemoglobin reducing system, reference concentrations of selected electrolytes, a polyphosphate compound and antibiotics. The ampule is subsequently opened and the solution is equilibrated with a gas mixture having components which provide gasses in a physiological range to the solution. The solution is characterized by exhibiting properties similar to fresh blood subsequent to equilibration.

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