Specific binding assay technique

Details Specific binding assay technique Specific binding assay technique

1983-02-28

1987-02-24

Kepplinger, Esther M.

Chemistry: analytical and immunological testing

Thyroid hormone tests

436538, 436542, 436541, 436807, 436816, 436817, 436824, G01N 33537, G01N 33538

Patent

active

046457470

ABSTRACT:
The present invention relates to a simple and accurate method to be applied in immunoassay technique for separating the free ligand from the antibody-bound ligand.
The method involves the use of a solvent extraction operation which at equilibrium provides the formation of two distinct phases the free- and bound-fraction. The solvent to be utilized should be slightly water miscible or completely water immiscible, having the property of marked selective extraction power toward the gamma-labelled constituent to be determined. The method is applicable in a liquid-liquid system, a solid-liquid system and solid-solid system.
The method is useful for radioimmunoassay, free radical assay or metallo-immunoassay, being most versatile having also the advantage that as a result of the free ligand being extracted into the solvent, the determination of the quantity of gamma-labelled substance in the free and/or bound fraction compares very favorably with the known prior art methods in immunoassays.

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Jowett et al, Clin. Sci Mol. Med., 45: 607-623 (1973).
Parker, Radioimmunoassay of Biologically Active Compounds, Prentice-Hall, Inc., Englewood Cliffs, N.J., 68-71 (1976).
Cason, Laboratory Text in Organic Chemistry, 2nd edition, Prentice-Hall, Inc., Englewood Cliffs, N.J., 259-261 (1962).

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